公开(公告)号：US3634196A

公开(公告)日：1972-01-11

申请号：US3634196D

申请日：1968-12-26

Applicant: BAYER AG

Inventor： WAGNER OTTO ,  BAUER KLAUS ,  KAUFMANN WILFRIED ,  RAUENBUSCH ERICH ,  ARENS ALFRED ,  IRION ECKARD

IPC: C12N9/82 ,  C07G7/02

CPC classification number: C12N9/82 ,  Y10S435/815 ,  Y10S435/849

Abstract: Crude L-asparaginase is freed of pyrogens by contacting solutions of the same containing weak buffers with diethylaminoethyl dextran gels. The process can be carried out according to batch techniques or in a continuous manner by using a chromatographic column.

Abstract translation: 通过将含有弱缓冲液的溶液与二乙基氨基乙基葡聚糖凝胶接触来解决热L-天冬酰胺酶，释放热原。 该方法可以根据间歇技术或通过使用色谱柱以连续方式进行。