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    Process for the extraction of l-asparaginase
    1.
    发明授权
    Process for the extraction of l-asparaginase 失效
    提取L-阿糖胞苷的方法

    公开(公告)号：US3622461A

    公开(公告)日：1971-11-23

    申请号：US3622461D

    申请日：1968-12-26

    Applicant: BAYER AG

    Inventor： WAGNER OTTO ,  BAUER KLAUS ,  KAUFMANN WILFRIED ,  RAUENBUSCH ERICH ,  ARENS ALFRED ,  IRION ECKART

    IPC: C12N9/82 ,  C07G7/028

    CPC classification number: C12N9/82 ,  Y10S435/816 ,  Y10S435/849

    Abstract: From a culture of E. coli the bacterial mass is flocculated by addition of an organic base or a salt thereof having an amino function and a molecular weight above 1,000. The flocculated cells are centrifuged, resuspended in water and precipitated by a water-miscible solvent, such as acetone. The cells which are simultaneously opened by the previous step are centrifuged away and again resuspended in water, whereby the enzyme is extracted from the cells. By addition of the organic base, mentioned above, to the suspension, the extracted cells, nucleic acids and ballast proteins are precipitated and are removed by centrifugation. The L-asparaginase is precipitated from the cell-free solution by addition of acetone.

    Water-insoluble peptide materials
    5.
    发明授权
    Water-insoluble peptide materials 失效
    水不溶性肽材料

    公开(公告)号：US3871964A

    公开(公告)日：1975-03-18

    申请号：US34545273

    申请日：1973-03-27

    Applicant: BAYER AG

    Inventor： HUPER FRITZ ,  RAUENBUSCH ERICH ,  SCHMIDT-KASTNER GUNTER ,  BOMER BRUNO ,  BARTL HERBERT

    IPC: A61K38/46 ,  C07K17/08 ,  C08F8/00 ,  C08F8/30 ,  C08F20/20 ,  C12N11/08 ,  C07G7/02

    CPC classification number: C12N11/08 ,  C08F8/00 ,  C08F20/20 ,  C08F20/02

    Abstract: Polypeptide materials such as enzymes are rendered waterinsoluble by bonding to a cross-linked copolymer produced by copolymerizing of (A) about 0.1 to 30 wt. % of at least one Alpha , Beta -monoolefinically unsaturated dicarboxylic acid anhydride having about 4 to 9 carbon atoms; (B) about 35 to 90 wt % of at least one di-and/or poly(meth)acrylate of a dial and/or polyol; and (C) about 5 to 60 wt % of at least one hydrophilic monomer.

    Abstract translation: 通过与（A）约0.1〜30重量％的共聚而制成的交联共聚物结合，使多肽材料如酶变得不溶于水。 ％的至少一种具有约4至9个碳原子的α，β-单烯属不饱和二羧酸酐; （B）约35至90重量％的至少一种表面和/或多元醇的二 - 和/或聚（甲基）丙烯酸酯; 和（C）约5至60重量％的至少一种亲水性单体。

    Process for the stabilization and purification of l-asparaginase
    6.
    发明授权
    Process for the stabilization and purification of l-asparaginase 失效
    L-ASPARAGINASE的稳定和纯化方法

    公开(公告)号：US3650902A

    公开(公告)日：1972-03-21

    申请号：US3650902D

    申请日：1969-05-28

    Applicant: BAYER AG

    Inventor： WAGNER OTTO ,  BAUER KLAUS ,  KAUFMANN WILFRIED ,  RAUENBUSCH ERICH ,  ARENS ALFRED ,  IRION ECKART

    IPC: C12N9/82 ,  C12N9/96 ,  C07G7/02

    CPC classification number: C12N9/96 ,  C12N9/82 ,  Y10S435/816 ,  Y10S435/849

    Abstract: L-asparaginase is obtained in a highly purified, pyrogen free and stabilized form from a crude aqueous solution thereof, by adding to said solution an amino acid, such as glycine, adjusting the pH of the solution to from 7.0 to 9.2, preferably in the presence of a lower aliphatic alcohol, such as methanol, then heating the solution for a period of from 1 to 5 days at a temperature up to 65* C., preferably between 40* C. to 61* C., thereby denaturizing the inactive accompanying proteins without affecting the L-asparaginase. The precipitated, denatured proteins are then removed, as by centrifuge, the enzyme enriched solution then adjusted to a pH between 4.5 and 5.5 and fractionally precipitated with a 50 percent polyethylene glycol solution, the precipitate then washed with acetone and dried.

    Abstract translation: 通过向所述溶液中加入氨基酸，例如甘氨酸，将溶液的pH调节至7.0至9.2，优选地在所述溶液中，优选地在所述溶液中加入L-天冬酰胺酶， 存在低级脂族醇如甲醇，然后在高达65℃，优选40℃至61℃的温度下将溶液加热1至5天，从而使惰性 伴随蛋白质而不影响L-天冬酰胺酶。 然后通过离心除去沉淀的变性蛋白质，然后通过离心将富酶溶液调节至4.5至5.5之间的pH并用50％聚乙二醇溶液分级沉淀，然后用丙酮洗涤并干燥。

    Separation and purification of alpha-amylase
    7.
    发明授权
    Separation and purification of alpha-amylase 失效
    ALPHA-AMYLASE的分离和纯化

    公开(公告)号：US3926730A

    公开(公告)日：1975-12-16

    申请号：US44372374

    申请日：1974-02-19

    Applicant: BAYER AG

    Inventor： HUPER FRITZ ,  RAUENBUSCH ERICH

    IPC: C12N9/26 ,  C07G7/02

    CPC classification number: C12N9/2414 ,  Y02P20/582 ,  Y10S435/815

    Abstract: Alpha -Amylase is separated from a liquid which contains Alpha -amylase and foreign protein impurities according to a process which comprises adsorbing the Alpha -amylase from the liquid onto a carrier-bound Alpha -amylase inhibitor to form an enzyme inhibitor complex and separating the complex from the liquid. Purified Alpha -amylase substantially free from foreign protein impurities may be obtained by washing the complex and desorbing the Alpha -amylase from the carrier-bound inhibitor.

    Water-insoluble peptide materials
    8.
    发明授权
    Water-insoluble peptide materials 失效
    水不溶性肽材料

    公开(公告)号：US3910825A

    公开(公告)日：1975-10-07

    申请号：US34579273

    申请日：1973-03-28

    Applicant: BAYER AG

    Inventor： HUPER FRITZ ,  RAUENBUSCH ERICH ,  SCHMIDT-KASTNER GUNTER ,  BOMER BRUNO ,  BARTL HERBERT

    IPC: C08F222/04 ,  C07D499/42 ,  C08F8/00 ,  C08F20/20 ,  C12N11/08 ,  C12B1/00 ,  C07G7/02 ,  C12D13/02

    CPC classification number: C12N11/08 ,  C08F8/00 ,  C08F20/20 ,  Y10S530/816 ,  C08F20/02

    Abstract: Peptide materials such as enzymes and enzyme inhibitors are rendered water-insoluble by binding the peptide materials to a cross-linked copolymer formed by copolymerizing the following units: (A) about 0.1 to 50 weight percent of at least one Alpha , Beta -monoolefinically unsaturated dicarboxylic acid anhydride having about 4 to 9 carbon atoms and (B) about 99.9 to 50 weight percent of at least one di- and/or poly(meth) acrylate of a diol and/or a polyol.

    Abstract translation: 肽材料如酶和酶抑制剂通过将肽材料与通过共聚以下单元形成的交联共聚物结合而变成水不溶性：（A）约0.1至50重量％的至少一种α，β-单烯属不饱和 具有约4至9个碳原子的二羧酸酐和（B）约99.9至50重量％的二醇和/或多元醇的至少一种二 - 和/或聚（甲基）丙烯酸酯。

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