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    Process for the stabilization and purification of l-asparaginase
    1.
    发明授权
    Process for the stabilization and purification of l-asparaginase 失效
    L-ASPARAGINASE的稳定和纯化方法

    公开(公告)号：US3650902A

    公开(公告)日：1972-03-21

    申请号：US3650902D

    申请日：1969-05-28

    Applicant: BAYER AG

    Inventor： WAGNER OTTO ,  BAUER KLAUS ,  KAUFMANN WILFRIED ,  RAUENBUSCH ERICH ,  ARENS ALFRED ,  IRION ECKART

    IPC: C12N9/82 ,  C12N9/96 ,  C07G7/02

    CPC classification number: C12N9/96 ,  C12N9/82 ,  Y10S435/816 ,  Y10S435/849

    Abstract: L-asparaginase is obtained in a highly purified, pyrogen free and stabilized form from a crude aqueous solution thereof, by adding to said solution an amino acid, such as glycine, adjusting the pH of the solution to from 7.0 to 9.2, preferably in the presence of a lower aliphatic alcohol, such as methanol, then heating the solution for a period of from 1 to 5 days at a temperature up to 65* C., preferably between 40* C. to 61* C., thereby denaturizing the inactive accompanying proteins without affecting the L-asparaginase. The precipitated, denatured proteins are then removed, as by centrifuge, the enzyme enriched solution then adjusted to a pH between 4.5 and 5.5 and fractionally precipitated with a 50 percent polyethylene glycol solution, the precipitate then washed with acetone and dried.

    Abstract translation: 通过向所述溶液中加入氨基酸，例如甘氨酸，将溶液的pH调节至7.0至9.2，优选地在所述溶液中，优选地在所述溶液中加入L-天冬酰胺酶， 存在低级脂族醇如甲醇，然后在高达65℃，优选40℃至61℃的温度下将溶液加热1至5天，从而使惰性 伴随蛋白质而不影响L-天冬酰胺酶。 然后通过离心除去沉淀的变性蛋白质，然后通过离心将富酶溶液调节至4.5至5.5之间的pH并用50％聚乙二醇溶液分级沉淀，然后用丙酮洗涤并干燥。

    Process for the extraction of l-asparaginase
    4.
    发明授权
    Process for the extraction of l-asparaginase 失效
    提取L-阿糖胞苷的方法

    公开(公告)号：US3622461A

    公开(公告)日：1971-11-23

    申请号：US3622461D

    申请日：1968-12-26

    Applicant: BAYER AG

    Inventor： WAGNER OTTO ,  BAUER KLAUS ,  KAUFMANN WILFRIED ,  RAUENBUSCH ERICH ,  ARENS ALFRED ,  IRION ECKART

    IPC: C12N9/82 ,  C07G7/028

    CPC classification number: C12N9/82 ,  Y10S435/816 ,  Y10S435/849

    Abstract: From a culture of E. coli the bacterial mass is flocculated by addition of an organic base or a salt thereof having an amino function and a molecular weight above 1,000. The flocculated cells are centrifuged, resuspended in water and precipitated by a water-miscible solvent, such as acetone. The cells which are simultaneously opened by the previous step are centrifuged away and again resuspended in water, whereby the enzyme is extracted from the cells. By addition of the organic base, mentioned above, to the suspension, the extracted cells, nucleic acids and ballast proteins are precipitated and are removed by centrifugation. The L-asparaginase is precipitated from the cell-free solution by addition of acetone.

    Alkylsulphonyl-cobalamines
    7.
    发明授权
    Alkylsulphonyl-cobalamines 失效
    烷基苯酚 - 钴胺

    公开(公告)号：US3573276A

    公开(公告)日：1971-03-30

    申请号：US3573276D

    申请日：1968-10-10

    Applicant: BAYER AG

    Inventor： WAGNER OTTO

    IPC: C07H23/00 ,  C07D55/62

    CPC classification number: C07H23/00

    Abstract: ALKYLSULPHONYL-COBALAMINES ARE PRODUCED BY REACTING HYDROXO-COBALAMINE WITH ALKYL-SULPHINIC ACIDS AT AMBIENT TEMPERATURE OR AT SLIGHTLY ELEVATED TEMPERATURES. THEY MAY ALSO BE PREPARED BY REACTING CYANO-COBALAMINES WITH ALKYL-SULPHINIC ACIDS. THE ALKULSULPHONYLCOBALAMINES ARE USEFUL IN THE SAME MANNER AS VITAMIN B12 AND MAY BE ADMINISTERED IN THE SAME GENERAL DOSAGE RANGES AND BY THE SAME GENERAL ROUTES OF ADMINISTRATION.

    Plasminogen activator and isolation thereof from stromata of human erythrocytes
    8.
    发明授权
    Plasminogen activator and isolation thereof from stromata of human erythrocytes 失效
    血清激活因子和人类红细胞生成素的分离

    公开(公告)号：US3555000A

    公开(公告)日：1971-01-12

    申请号：US3555000D

    申请日：1968-02-20

    Applicant: BAYER AG

    Inventor： WAGNER OTTO

    IPC: A61K38/00 ,  C12N9/72 ,  C07G7/00 ,  C07G7/026

    CPC classification number: C12N9/6462 ,  A61K38/00 ,  C12Y304/21073 ,  Y10S530/829

    Abstract: A NEW PLASMINOGEN ACTIVATOR DISTINGUISHABLE FROM UROKINASE CAN BE ISOLATED FROM STROMATA OF HUMAN ERYTHROCYTES AT A PH OF FROM ABOUT 1 TO 4. THE PLASMINOGEN ACTIVATOR IS USEFUL FOR THE THERAPY AND PROPHYLAXIS OF THROMBOSES AND EMBOLISMS. THE PLASMINOGEN ACTIVATOR IS ADMINISTERED IN THE FORM OF AN INJECTABLE SOLUTION WHICH CAN BE PREPARED BY TECHNIQUES WHICH ARE PER SE KNOWN IN A THERAPEUTIC DOSAGEOF TE SAME ORDER OF MAGNITUDE AS UROINASE. IT HAS A MOLECULAR WEIGHT AFTER PROCESSING IN THE ULTRACENTRIFUGE BETWEEN ABOUT 20,000 AND 50,000. IT IS A PROTEIN, ACID STABLE TO PH 1 BUT IS ALMOST ENTIRELY DESTROYED AT PH 9-10 AT +4*C. FOR 5 HOURS. IT IS ACTIVATED OR CATALYZED BY SMALL AMOUNTS OF UROKINASE WHICH RAISED ITS ACTIVITY 20-100 FOLD.

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