公开(公告)号：US10221429B2

公开(公告)日：2019-03-05

申请号：US14773015

申请日：2014-03-06

Applicant: BAYER CROPSCIENCE LP ,  Athenix Corp.

Inventor： Rebecca Thayer ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Cheryl Peters ,  Kira Roberts ,  Leonardo Magalhaes ,  Ethan Dunn

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/02 ,  A01N37/46

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-74, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.

公开(公告)号：US20160017363A1

公开(公告)日：2016-01-21

申请号：US14773015

申请日：2014-03-06

Applicant: ATHENIX CORP. ,  BAYER CROPSCIENCE LP

Inventor： Rebecca Thayer ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Cheryl Peters ,  Kira Roberts ,  Leonardo Magalhaes ,  Ethan Dunn

IPC: C12N15/82 ,  A01N37/44 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-74, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-74所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-20所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US10221430B2

公开(公告)日：2019-03-05

申请号：US14910474

申请日：2014-08-08

Applicant: Athenix Corp.

Inventor： Rebecca Thayer ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Leonardo Magalhaes

IPC: C12N15/82 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO: 1 or 2, as well as variants and fragments thereof.

公开(公告)号：US20160185830A1

公开(公告)日：2016-06-30

申请号：US14910474

申请日：2014-08-08

Applicant: ATHENIX CORP

Inventor： Rebecca Thayer ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Leonardo Magalhaes

IPC: C07K14/325 ,  C12N15/82 ,  A01N63/02

CPC classification number: C12N15/8286 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO: 1 or 2, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3-6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或2所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20160201082A1

公开(公告)日：2016-07-14

申请号：US14910479

申请日：2014-08-08

Applicant: ATHENIX CORP.

Inventor： Kira Roberts ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Leonardo Magalhaes

IPC: C12N15/82 ,  A01N63/02 ,  C07K14/325

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-3, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了包含编码SEQ ID NO：2-3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子，以及其变体和片段 。

公开(公告)号：US10221431B2

公开(公告)日：2019-03-05

申请号：US14910479

申请日：2014-08-08

Applicant: Athenix Corp.

Inventor： Kira Roberts ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Leonardo Magalhaes

IPC: C12N15/82 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-3, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.