公开(公告)号：US10221430B2

公开(公告)日：2019-03-05

申请号：US14910474

申请日：2014-08-08

Applicant: Athenix Corp.

Inventor： Rebecca Thayer ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Leonardo Magalhaes

IPC: C12N15/82 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO: 1 or 2, as well as variants and fragments thereof.

公开(公告)号：US10093946B2

公开(公告)日：2018-10-09

申请号：US15102785

申请日：2014-12-08

Applicant: Athenix Corp.

Inventor： Duane Alan Lehtinen ,  Kimberly S. Sampson ,  Kira Roberts ,  Ethan Dunn

IPC: C07K14/325 ,  C12N15/82

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a Bacillus thuringiensis toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5-26, or the nucleotide sequence set forth in SEQ ID NO: 1-4, as well as variants and fragments thereof.

公开(公告)号：US20160185830A1

公开(公告)日：2016-06-30

申请号：US14910474

申请日：2014-08-08

Applicant: ATHENIX CORP

Inventor： Rebecca Thayer ,  Kimberly S. Sampson ,  Duane Lehtinen ,  Leonardo Magalhaes

IPC: C07K14/325 ,  C12N15/82 ,  A01N63/02

CPC classification number: C12N15/8286 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO: 1 or 2, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3-6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或2所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20150368305A1

公开(公告)日：2015-12-24

申请号：US14847162

申请日：2015-09-08

Applicant: Athenix Corp.

Inventor： Kimberly Sampson ,  Daniel Tomso

IPC: C07K14/325 ,  A01N37/46 ,  A01N63/02 ,  C12N15/82

CPC classification number: C07K14/325 ,  A01N37/46 ,  A01N63/02 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：13-30所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20140373195A1

公开(公告)日：2014-12-18

申请号：US14376944

申请日：2013-03-07

Applicant: Athenix Corp.

Inventor： Kimberly Sampson ,  Rebecca Thayer ,  Duane Lehtinen

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8286 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1 or 3, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列，以及其变体和片段 。

公开(公告)号：US20140051829A1

公开(公告)日：2014-02-20

申请号：US14057300

申请日：2013-10-18

Applicant: Athenix Corp.

Inventor： Nalini Manoj Desai ,  Jill Hinson ,  Deepa Balasubramanian ,  Kimberly S. Sampson ,  Daniel J. Tomso ,  Duane Alan Lehtinen ,  Nicholas B. Duck ,  Rong Guo ,  Volker Heinrichs

IPC: C07K14/195

CPC classification number: C07K14/195 ,  C07K14/22 ,  C07K16/1203 ,  C12N15/8285 ,  C12N15/8286 ,  C12Q1/6876 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列 ，以及其变体和片段。

公开(公告)号：US10647996B2

公开(公告)日：2020-05-12

申请号：US15960817

申请日：2018-04-24

Applicant: ATHENIX CORP.

Inventor： Nalini Manoj Desai ,  Volker Heinrichs ,  Duane Lehtinen

IPC: C12N15/82 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2, 3, 7-14 and 47, including biologically active variants and fragments thereof.

公开(公告)号：US09752158B2

公开(公告)日：2017-09-05

申请号：US14851442

申请日：2015-09-11

Applicant: ATHENIX CORP.

Inventor： Nalini Manoj Desai ,  Volker Heinrichs ,  Duane Lehtinen

IPC: C12N15/82 ,  C07K14/325 ,  A01N37/44 ,  A01N63/02 ,  C07K14/32

CPC classification number: C12N15/8286 ,  A01N37/44 ,  A01N63/02 ,  C07K14/32 ,  C07K14/325 ,  C07K2319/00 ,  C07K2319/24 ,  C12N15/8279 ,  C12N15/8285 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof.

公开(公告)号：US20160311865A1

公开(公告)日：2016-10-27

申请号：US15102785

申请日：2014-12-08

Applicant: ATHENIX CORP.

Inventor： Duane Alan Lehtinen ,  Kimberly S. Sampson ,  Kira Roberts ,  Ethan Dunn

IPC: C07K14/325 ,  C12N15/82 ,  A01N37/18

CPC classification number: C07K14/325 ,  C12N15/8286 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a Bacillus thuringiensis toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5-26, or the nucleotide sequence set forth in SEQ ID NO: 1-4, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含苏云金芽孢杆菌毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：5-26所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-4所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US09260487B2

公开(公告)日：2016-02-16

申请号：US14057250

申请日：2013-10-18

Applicant: Athenix Corp.

Inventor： Nalini Manoj Desai ,  Jill Hinson ,  Deepa Balasubramanian ,  Kimberly S. Sampson ,  Daniel J. Tomso ,  Duane Alan Lehtinen ,  Nicholas B. Duck ,  Rong Guo ,  Volker Heinrichs

IPC: C07K16/00 ,  C07K14/195 ,  C12Q1/68 ,  C12N15/82 ,  C07K14/22 ,  C07K16/12

CPC classification number: C07K14/195 ,  C07K14/22 ,  C07K16/1203 ,  C12N15/8285 ,  C12N15/8286 ,  C12Q1/6876 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.