公开(公告)号：US20210115436A1

公开(公告)日：2021-04-22

申请号：US17033470

申请日：2020-09-25

Applicant: BERKELEY LIGHTS, INC.

Inventor： Ravi K. RAMENANI ,  Duane SMITH ,  Jason M. McEWEN ,  Magali SOUMILLON

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/686

Abstract: Processes and kits are provided for producing sequence specific fragments of nucleic acid molecules, whether from a genome or transcriptome, where one end of the molecule is highly diverse and/or the full-length molecule, whether a gene or a mRNA, is too long for it to be sequenced using currently available sequencing methods. Methods of preparing a sequencing library configured for 5′ or 3′ anchored sequencing, wherein the opposing termini of the library molecules are differentially truncated, and methods of parallel sequencing such libraries are described.