公开(公告)号：US20240288442A1

公开(公告)日：2024-08-29

申请号：US18476798

申请日：2023-09-28

申请人： BERKELEY LIGHTS, INC.

发明人： Phillip J. M. ELMS ,  Preston Lock NG ,  Jonathan CHENG ,  Nathan J. VER HEUL ,  Jason King Mckenzie YEE ,  Hariharasudhan CHIRRA DINAKAR ,  Renee Lynn TOBIAS ,  Eric K. SACKMANN ,  Wesley Arthur ZINK ,  Hector D. NEIRA-QUINTERO

IPC分类号： G01N33/68

CPC分类号： G01N33/6854 ,  G01N2458/00

摘要： Methods for identifying a cell population secreting a biomolecule with desirable attributes are disclosed. The desirable attributes can include, for example, quantity and quality (e.g., minimal aggregation and/or desired configuration). Cell populations identified by the disclosed methods are more likely to successfully scale during production. The methods can include assessing multiple domains/binding sites of a complex biomolecule and/or the formation of aggregates by the biomolecules. Methods of assessing a secretion level for biomolecules having a wide range of molecular weights and method for enhancing loading of cells into chambers of a microfluidic device. The labelling of viable cells and/or cells actively expressing a biomolecule of interest, for example, can permit selection and subsequent analysis of the cells most likely to successfully expand and express the biomolecule of interest, thereby reducing effort, risk, and cost associated with the screening of cells and increase the probability of identifying cell lines that are optimal producers of biomolecules of interest.

公开(公告)号：US20240027396A1

公开(公告)日：2024-01-25

申请号：US18324026

申请日：2023-05-25

申请人： BERKELEY LIGHTS, INC.

发明人： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC分类号： G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

CPC分类号： G01N27/44791 ,  B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  B01L2400/0454

摘要： Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US20230323433A1

公开(公告)日：2023-10-12

申请号：US18178927

申请日：2023-03-06

申请人： Berkeley Lights, Inc.

发明人： Matthew Asuka Kubit ,  Joshua David Mast ,  John Junyeon Kim ,  Alexander Gerald Olson ,  Preston Lock Ng ,  Arlvin Louis Ellefson ,  Shruthi Sreedhar Kubatur ,  Vincent Haw Tien Pai ,  Minha Park ,  Po-Yuan Tung ,  Jason C. Briggs ,  Patrick N. Ingram ,  Katrine Elise Dailey ,  Maryam Shansab ,  Jason M. McEwen ,  Adrienne T. Higa ,  Hongye Zhou ,  Zhen Hu ,  John A. Tenney

IPC分类号： C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6804

CPC分类号： C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6844 ,  C12Q2600/16

摘要： Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.

公开(公告)号：US20230282313A1

公开(公告)日：2023-09-07

申请号：US17971400

申请日：2022-10-21

申请人： BERKELEY LIGHTS, INC.

发明人： Darshan Thaker ,  Keith J. Breinlinger ,  Vincent Haw Tien Pai ,  Christoph Andreas Neyer ,  Thomas M. Vetterli ,  Hayley M. Bennett ,  Elisabeth Marie Walczak ,  Alexander Gerald Olson ,  Wesley Arthur Zink ,  John A. Tenney ,  Oleksandr Tokmakov ,  Igor Fastnacht ,  Yuriy Nicheporuk ,  Andriy Koval ,  Khrystyna Andres ,  Alona Kostenko

IPC分类号： G16B40/20 ,  G16B45/00 ,  G16B30/20 ,  B01L3/00 ,  G01N15/14

CPC分类号： G16B40/20 ,  G16B45/00 ,  G16B30/20 ,  B01L3/502784 ,  G01N15/1463 ,  B01L2200/027

摘要： Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

公开(公告)号：US11731129B2

公开(公告)日：2023-08-22

申请号：US17357372

申请日：2021-06-24

申请人： BERKELEY LIGHTS, INC.

发明人： Paul M. Lundquist ,  Paul M. Lebel ,  Phillip Ronald Thomas Jess

IPC分类号： B01L3/00 ,  B01L7/00 ,  G01J3/02 ,  G01J3/10 ,  G01J3/44 ,  G01N21/31 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G01N1/40

CPC分类号： B01L3/502715 ,  B01L7/00 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/10 ,  G01J3/4406 ,  G01N1/40 ,  G01N21/31 ,  G01N21/6458 ,  G02B21/06 ,  G02B21/16 ,  G02B21/361 ,  B01L2200/147 ,  B01L2300/046 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0424

摘要： The present disclosure relates to an optical apparatus for imaging and/or manipulating micro-objects in a microfluidic device, such as a light-actuated microfluidic (LAMF) device, and related systems and methods. The optical apparatus can comprise a structured light modulator, a first and a second tube lens, an objective lens, a dichroic beam splitter, and an image sensor. The structured light modulator can be configured to receive unstructured light beams and transmit structured light beams for illuminating micro-objects located within an enclosure of the microfluidic device and/or selectively activating one or more of a plurality of dielectrophoresis (DEP) electrodes of the microfluidic device. The image light beams received by the image sensor can be used to form an image of at least a portion of the microfluidic device.

公开(公告)号：US11639495B2

公开(公告)日：2023-05-02

申请号：US16455118

申请日：2019-06-27

申请人： The Regents of the University of California ,  Berkeley Lights, Inc.

发明人： Alexander Marson ,  Gregory G. Lavieu ,  Annamaria Mocciaro ,  Theodore L. Roth ,  Magali Soumillon ,  Hayley M. Bennett

IPC分类号： C12N5/0783 ,  B01L3/00 ,  C12N15/10

摘要： Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.

公开(公告)号：US20220356429A1

公开(公告)日：2022-11-10

申请号：US17743318

申请日：2022-05-12

申请人： Berkeley Lights, Inc.

发明人： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC分类号： C12M3/06 ,  C12M1/00

摘要： Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US11376591B2

公开(公告)日：2022-07-05

申请号：US16893885

申请日：2020-06-05

申请人： Berkeley Lights, Inc.

发明人： Troy A. Lionberger ,  Brandon R. Bruhn ,  John A. Tenney ,  Eric D. Hobbs

IPC分类号： B01L3/00 ,  G01N27/447 ,  B03C5/00 ,  B03C5/02

摘要： Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

公开(公告)号：US20220130158A1

公开(公告)日：2022-04-28

申请号：US17495728

申请日：2021-10-06

申请人： Berkeley Lights, Inc.

发明人： Hansohl E. KIM ,  John A. TENNEY ,  Joshua F. SLOCUM

IPC分类号： G06V20/69 ,  G06T7/73 ,  G06T7/246 ,  G06K9/62 ,  G06T1/00 ,  G06V10/88

摘要： Methods are provided for the automated detection and/or counting of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

公开(公告)号：US11305283B2

公开(公告)日：2022-04-19

申请号：US16509166

申请日：2019-07-11

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.