公开(公告)号：US20210115436A1

公开(公告)日：2021-04-22

申请号：US17033470

申请日：2020-09-25

Applicant: BERKELEY LIGHTS, INC.

Inventor： Ravi K. RAMENANI ,  Duane SMITH ,  Jason M. McEWEN ,  Magali SOUMILLON

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/686

Abstract: Processes and kits are provided for producing sequence specific fragments of nucleic acid molecules, whether from a genome or transcriptome, where one end of the molecule is highly diverse and/or the full-length molecule, whether a gene or a mRNA, is too long for it to be sequenced using currently available sequencing methods. Methods of preparing a sequencing library configured for 5′ or 3′ anchored sequencing, wherein the opposing termini of the library molecules are differentially truncated, and methods of parallel sequencing such libraries are described.

公开(公告)号：US20210349075A1

公开(公告)日：2021-11-11

申请号：US17241865

申请日：2021-04-27

Applicant: BERKELEY LIGHTS, INC.

Inventor： Yelena BRONEVETSKY ,  Annamaria MOCCIARO ,  Guido K. STADLER ,  Peter J. BEEMILLER ,  Natalie C. MARKS ,  Duane SMITH ,  Vincent Haw Tien PAI ,  Jason M. MCEWEN ,  Amanda L. GOODSELL ,  John A. TENNEY ,  Thomas M. VETTERLI ,  Hansohl E. Kim

IPC: G01N33/50 ,  B01L3/00

Abstract: Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.