公开(公告)号：US11972583B2

公开(公告)日：2024-04-30

申请号：US17426885

申请日：2019-01-31

Applicant: BGI SHENZHEN

Inventor： Jin-Jin Shen ,  Da-Wei Li ,  Yang-Bao Liu ,  Ge Feng ,  Mei Li ,  Yu-Xiang Li

IPC: G06T7/33 ,  C12Q1/6869 ,  G01N21/64 ,  G06T7/66 ,  G06T7/73

CPC classification number: G06T7/337 ,  C12Q1/6869 ,  G01N21/6458 ,  G01N21/6486 ,  G06T7/66 ,  G06T7/73 ,  G06T2207/10064 ,  G06T2207/30072 ,  G06T2207/30168

Abstract: The present disclosure provides a fluorescence image registration method, the method includes: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring a position of a minimum value of a sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels; if the fluorescence image meets the preset standard, correcting positions of the defective pixels according to positions of the non-defective pixels; acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points.