公开(公告)号：US11972583B2

公开(公告)日：2024-04-30

申请号：US17426885

申请日：2019-01-31

Applicant: BGI SHENZHEN

Inventor： Jin-Jin Shen ,  Da-Wei Li ,  Yang-Bao Liu ,  Ge Feng ,  Mei Li ,  Yu-Xiang Li

IPC: G06T7/33 ,  C12Q1/6869 ,  G01N21/64 ,  G06T7/66 ,  G06T7/73

CPC classification number: G06T7/337 ,  C12Q1/6869 ,  G01N21/6458 ,  G01N21/6486 ,  G06T7/66 ,  G06T7/73 ,  G06T2207/10064 ,  G06T2207/30072 ,  G06T2207/30168

Abstract: The present disclosure provides a fluorescence image registration method, the method includes: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring a position of a minimum value of a sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels; if the fluorescence image meets the preset standard, correcting positions of the defective pixels according to positions of the non-defective pixels; acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points.

公开(公告)号：US12277468B2

公开(公告)日：2025-04-15

申请号：US18651099

申请日：2024-04-30

Applicant: BGI SHENZHEN

Inventor： Wenwei Zhang ,  Mei Li ,  Weimao Wang ,  Yuxiang Li ,  Xun Xu

IPC: G06K7/08 ,  G01N33/543 ,  G06T5/77 ,  G06T7/00 ,  G06T7/13 ,  G06T7/62 ,  G06T7/73

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).

公开(公告)号：US12276809B2

公开(公告)日：2025-04-15

申请号：US17769428

申请日：2020-03-09

Applicant: BGI SHENZHEN

Inventor： Jielei Ni ,  Ming Ni ,  Fan Zhou ,  Zeyu Su ,  Ke Ji ,  Dong Wei ,  Mengzhe Shen ,  Yuanqing Liang ,  Mei Li ,  Xun Xu

IPC: G01N21/00 ,  C12Q1/6869 ,  G01N21/64 ,  G02B21/14 ,  G02B21/16 ,  G02B21/36 ,  G02B27/28 ,  G02B27/58

Abstract: Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

公开(公告)号：US20240281625A1

公开(公告)日：2024-08-22

申请号：US18651099

申请日：2024-04-30

Applicant: BGI SHENZHEN

Inventor： Wenwei Zhang ,  Mei Li ,  Weimao Wang ,  Yuxiang Li ,  Xun Xu

IPC: G06K7/08 ,  G01N33/543 ,  G06T5/77 ,  G06T7/00 ,  G06T7/13 ,  G06T7/62 ,  G06T7/73

CPC classification number: G06K7/087 ,  G01N33/54326 ,  G06T5/77 ,  G06T7/0002 ,  G06T7/13 ,  G06T7/62 ,  G06T7/73 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30204

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).