公开(公告)号：US20240367143A1

公开(公告)日：2024-11-07

申请号：US18653214

申请日：2024-05-02

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： SHAWN DARNALL ,  BRIAN PANGANIBAN ,  DANNI WANG ,  CHRISTOPHER BELISLE ,  ZACHARY CLAUSS

IPC: B01J20/04 ,  B01D15/08 ,  B01J20/28 ,  B01J20/282 ,  B01J20/30 ,  B01J20/32 ,  B01J20/34

Abstract: Provided herein is a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with hydroxyapatite (IA). Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Thus this disclosure provides a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with I-IA. Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Methods of preparing the HA-coated substrate are also provided.

公开(公告)号：US20240367161A1

公开(公告)日：2024-11-07

申请号：US18654043

申请日：2024-05-03

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： PEYTON RUSSELBURG ,  BRIAN PANGANIBAN ,  DANNI WANG ,  JOSEPH POBLETE ,  ALEXANDER POLGAR

IPC: B01J43/00 ,  B01D15/38 ,  B01J47/02 ,  C07K1/22

Abstract: The subject invention pertains to multimodal chromatography resins comprising anionic and cationic ligands on each resin particle and methods of synthesizing the multimodal chromatography resin by contacting a base with quaternary amines and/or tertiary amines on the surface of a substrate. The multimodal resins can be tuned by changing the ratios of cation to anions. The multimodal chromatography resins can be used for purifying proteins.

公开(公告)号：US20240367142A1

公开(公告)日：2024-11-07

申请号：US18652908

申请日：2024-05-02

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： BRIAN PANGANIBAN ,  SHAWN DARNALL ,  DANNI WANG ,  CHRISTOPHER BELISLE

IPC: B01J20/04 ,  B01J20/282 ,  C01B25/32

Abstract: Provided herein is a chromatography media composed of a particulate substrate (such as beads or other particulate substrates) that is coated with hydroxyapatite (HA) and in which the particulate substrate used as the base upon which calcium phosphate (CaP) is formed is treated with heat to remove the substrate, convert the CaP to HA, and form the desired HA microstructure so as to form a templated porous HA substrate (TPHA substrate). Also provided is a chromatography media (optionally contained within a column) comprising a TPHA substrate (e.g., a plurality of TPHA particles). The TPHA particles are porous or macroporous and uses thereof.

公开(公告)号：US20240367141A1

公开(公告)日：2024-11-07

申请号：US18652860

申请日：2024-05-02

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： BRIAN PANGANIBAN ,  SHAWN DARNALL ,  DANNI WANG ,  CHRISTOPHER BELISLE

IPC: B01J20/04 ,  B01D15/20 ,  B01D15/42 ,  B01J20/282 ,  B01J20/30 ,  B01J20/32

Abstract: This disclosure relates to particulate hydroxyapatite (HA)-coated substrates, methods of making particulate HA-coated substrates, and methods of using particulate HA-coated substrates. Thus, the disclosure provides chromatography media composed of a particulate substrate (such as polymer beads or other particulate substrates) that is coated with HA. Also provided is a chromatography media (optionally contained within a column) comprising a HA-coated substrate (e.g., a plurality of beads or another particulate substrate that is coated with HA). The beads or particulate substrate can be a porous or non-porous substrate.