公开(公告)号：US20210322988A1

公开(公告)日：2021-10-21

申请号：US17271010

申请日：2019-09-23

Applicant: BIOMÉRIEUX ,  BIOASTER

Inventor： Christophe VEDRINE ,  Yacine BOUNAB

IPC: B01L3/00 ,  G01N33/58 ,  G01N33/543 ,  G01N33/50 ,  G01N21/64

Abstract: The invention provides for a microfluidic droplet-based assay process and apparatus, wherein the process comprises the step of creating several assay droplets (18) containing paramagnetic particles (22, 22′), detectable markers (24), and at least one biological cell (10), wherein the paramagnetic particles (22, 22′) and the detectable markers (24) are able to bind with a target analyte (16) released by the cell, wherein the process includes storing several assay droplets along a horizontal storage plane, includes the generation of a magnetic field through the stored assay droplets, and includes detecting a physical characteristic of the detectable markers contained in a given assay droplet by optical imaging of the detectable markers, characterized in that the generation of a magnetic field generates a magnetic field such that the paramagnetic particles are attracted towards a lateral and inferior portion of the given stored assay droplet in which they are contained.

公开(公告)号：US20210373004A1

公开(公告)日：2021-12-02

申请号：US17294327

申请日：2019-11-18

Applicant: BIOASTER

Inventor： Patrick LECINE ,  Christophe VEDRINE ,  Adrien LUGARI

IPC: G01N33/543 ,  G01N33/68

Abstract: A support for a multiplex binding experiment is functionalized with at least two different polypeptides. The polypeptides are provided in a reaction mixture along with their cognate binding partners. The polypeptides have high affinity for their cognate binding partners provided in the reaction mixture. The polypeptides and their cognate binding partners can be used in immunoassays.