公开(公告)号：US20150353883A1

公开(公告)日：2015-12-10

申请号：US14760182

申请日：2014-01-10

Applicant: BIOGEN MA INC.

Inventor： Alan GILBERT ,  Kyle MCELEARNEY ,  Terrence Michael DOBROWSKY ,  Rashmi Rohit KSHIRSAGAR

IPC: C12N5/00 ,  C12P21/00 ,  C07K14/755

CPC classification number: C12N5/0018 ,  C07K14/755 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2511/00 ,  C12P21/00

Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.

Abstract translation: 本发明涉及包含硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物的细胞培养基及其使用方法。 本发明还涉及在大规模细胞培养物中生产感兴趣的蛋白质的方法，其包括用硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物补充细胞培养物。

公开(公告)号：US20170247655A1

公开(公告)日：2017-08-31

申请号：US15425056

申请日：2017-02-06

Applicant: BIOGEN MA INC.

Inventor： Rashmi Rohit KSHIRSAGAR ,  Alan Gilbert

IPC: C12N5/07 ,  A61K39/395 ,  C12M1/02

CPC classification number: C12P21/00 ,  A61K39/39591 ,  A61K2039/505 ,  C07K1/1133 ,  C07K16/00 ,  C07K2317/40 ,  C07K2317/41 ,  C07K2317/94 ,  C12N5/0018 ,  C12N2500/34

Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.