公开(公告)号：US12031152B2

公开(公告)日：2024-07-09

申请号：US17341220

申请日：2021-06-07

申请人： Upside Foods, Inc.

发明人： Kalle Lukyan Johnson ,  Patricia R. Benton ,  Cameron Gentry Copeland ,  Sukhdeep Singh Dhadwar ,  Meri Teresa Firpo ,  Marie Elizabeth Gibbons ,  Stephen K. Hsu ,  Robert Sierra ,  Kevin John Kayser

IPC分类号： C12N5/071

CPC分类号： C12N5/0602 ,  C12N2500/14 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33

摘要： Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.

公开(公告)号：US20240150723A1

公开(公告)日：2024-05-09

申请号：US18283614

申请日：2022-03-22

申请人： Biogen MA Inc.

发明人： Taylor Forte ,  Christoper Kwiatkowski ,  Greg Evangelist ,  Taylor Roland ,  Krishnakumar Malu

IPC分类号： C12N5/071

CPC分类号： C12N5/0682 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2523/00

摘要： In some instances, the application provides methods of culturing host cells comprising a gene encoding a recombinant N protein in a cell culture medium, wherein the cell culture medium comprises: (i) iron at a concentration of less than 1200 μM; and (ii) citrate at a concentration of less than 2400 μM. In some instances, the methods comprise culturing host cells expressing a recombinant protein in a cell culture medium at a first temperature; and decreasing the first temperature to a second temperature. In some instances, the methods comprise purifying the recombinant protein by a carbon depth filtration.

公开(公告)号：US20240093143A1

公开(公告)日：2024-03-21

申请号：US18497162

申请日：2023-10-30

申请人： GENENTECH, INC.

发明人： Natarajan Vijayasankaran ,  Steven J. Meier ,  Melissa S. Mun ,  Sharat Varma ,  Yi Yang ,  Boyan Zhang ,  Silvana R. Arevalo ,  Martin Gawlitzek ,  Veronica Carvalhal

IPC分类号： C12N5/00 ,  C07K16/00 ,  C12P21/00

CPC分类号： C12N5/0018 ,  C07K16/00 ,  C12N5/005 ,  C12P21/00 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/39

摘要： Cell culture media, such as chemically defined cell culture media, are provided, as are methods of using the media for cell growth (i.e., cell culture) and polypeptide (e.g., antibody) production. Compositions comprising polypeptides produced by the methods are also provided.

公开(公告)号：US20230322866A1

公开(公告)日：2023-10-12

申请号：US17898870

申请日：2022-08-30

申请人： Suncor Energy Inc.

发明人： Samuel S. Slattery ,  Daniel J. Giguere ,  Martin Flatley ,  Gregory B. Gloor ,  David R. Edgell

IPC分类号： C12N15/79 ,  A61K39/215 ,  C07K14/165 ,  G01N33/543 ,  C12N1/12

CPC分类号： C07K14/165 ,  A61K39/215 ,  C12N1/12 ,  C12N15/79 ,  G01N33/54388 ,  C12N2500/24 ,  C12N2500/42

摘要： Phosphate-regulated expression of recombinant glycoprotein antigens and other recombinant proteins in diatoms is described herein. More specifically, described herein is the expression and purification of glycosylated, immunogenic, and serologically active receptor-binding domain (RBD) of the SARS-CoV-2 spike protein, as well as SARS-CoV-2 nucleocapsid protein, in the marine pennate diatom Phaeodactylum tricornutum, as well as a functional lateral flow assay-based diagnostic device based on the produced recombinant RBD and nucleocapsid protein. Also described herein is the use of phosphate/iron levels in culture media to regulate expression/secretion of recombinant proteins under control of an HASP1 promoter in P. tricornutum or other suitable host cells. Also described herein is a method for increasing the expression/secretion of a recombinant protein by engineering the recombinant protein to lack a Tobacco Etch Virus (TEV) protease cleavage site.

公开(公告)号：US20230250399A1

公开(公告)日：2023-08-10

申请号：US18302043

申请日：2023-04-18

申请人： BRISTOL-MYERS SQUIBB COMPANY

发明人： Jun Tian

IPC分类号： C12N5/071 ,  C12P21/00 ,  C12N5/00

CPC分类号： C12N5/0682 ,  C12P21/005 ,  C12N5/0031 ,  C12N2500/22 ,  C12N2500/32 ,  C12N2510/02 ,  C12N2500/24

摘要： In accordance with the present invention, CHO cells expressing a recombinant polypeptide of interest are grown in media where the amino acids, vitamins, phosphate, lipids and/or antioxidant optimization is utilized to manipulate and/or control the protein quality attributes of the polypeptides. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical compositions.

公开(公告)号：US20190231817A1

公开(公告)日：2019-08-01

申请号：US15772224

申请日：2016-10-28

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Gay M. CROOKS ,  Amélie MONTEL-HAGEN ,  Christopher SEET

IPC分类号： A61K35/17 ,  C12N5/00 ,  C12N5/0783 ,  C12N5/074 ,  C12N5/0735 ,  C12N5/0775 ,  C12N5/077 ,  A61P35/00

CPC分类号： A61K35/17 ,  A61P35/00 ,  C07K14/705 ,  C07K2317/622 ,  C07K2319/03 ,  C12N5/00 ,  C12N5/0037 ,  C12N5/0062 ,  C12N5/0606 ,  C12N5/0636 ,  C12N5/0663 ,  C12N5/0669 ,  C12N5/0696 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/15 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/2321 ,  C12N2501/25

摘要： Methods and composition for production of T cells are provided. Also provided are therapeutic methods using engineered T cells. For example, in certain aspects methods include preparing three dimensional cell culture compositions comprising stroma cells and hematopoietic stem or progenitor cells in a serum-free medium for producing T cells.

公开(公告)号：US20180155687A1

公开(公告)日：2018-06-07

申请号：US15645004

申请日：2017-07-10

申请人： Whitehead Institute for Biomedical Research ,  The Brigham and Women's Hospital, Inc.

发明人： Tan A. Ince ,  Robert A. Weinberg

IPC分类号： C12N5/071 ,  C12N15/85

CPC分类号： C12N5/0631 ,  C12N15/85 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/36 ,  C12N2500/42 ,  C12N2500/60 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395

摘要： The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate.The invention also provides methods to transform normal primary cells so cultured into “cancer stem cells.” The genetically defined cancer stem cell tumor model mimics the behavior of the disease closely, e.g., the cells are invasive, hormone responsive and metastatic when injected into mice. The tumor cells express genes that are specific to cancer stem cells identified in patient samples.

公开(公告)号：US20180044405A1

公开(公告)日：2018-02-15

申请号：US15591022

申请日：2017-05-09

申请人： Baxalta Incorporated ,  Baxalta GmbH

发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Daniel Fleischanderl ,  Gregor Bramberger

IPC分类号： C07K14/755 ,  A61K38/36 ,  A61K9/00 ,  C12P21/00 ,  C12N9/64 ,  A61K38/37 ,  A61K38/00

CPC分类号： C07K14/755 ,  A61K9/0019 ,  A61K38/00 ,  A61K38/36 ,  A61K38/37 ,  C12N9/6489 ,  C12N2500/24 ,  C12N2511/00 ,  C12P21/00 ,  C12Y304/24087

摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

公开(公告)号：US09758568B2

公开(公告)日：2017-09-12

申请号：US14942771

申请日：2015-11-16

申请人： Baxalta Incorporated ,  Baxalta GmbH

发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Artur Mitterer

IPC分类号： C12N1/00 ,  C07K14/755 ,  C12N7/00 ,  C12N5/00

CPC分类号： C07K14/755 ,  C12N5/0018 ,  C12N5/005 ,  C12N7/00 ,  C12N2500/05 ,  C12N2500/10 ,  C12N2500/20 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/50 ,  C12N2710/24151

摘要： The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.

公开(公告)号：US20170058259A1

公开(公告)日：2017-03-02

申请号：US15024590

申请日：2014-09-24

申请人： THE UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC

发明人： Tan A. Ince

IPC分类号： C12N5/071 ,  C12N5/09

CPC分类号： C12N5/0631 ,  C12N5/0693 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/392

摘要： A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.

摘要翻译： 包含三磷酸腺苷的细胞培养基; 载体蛋白; 胆固醇，亚油酸和硫辛酸; 谷胱甘肽 至少一个核苷酸补救途径前体碱基; 磷酸乙醇胺; 硒; 转铁蛋白 三碘甲状腺原氨酸 全反式视黄酸（ATRA）和维生素C; 锌，镁和铜; 增加细胞内cAMP的药物; 表皮生长因子（EGF）; 氢化可的松 胰岛素; 和木炭剥离的胎牛血清，其中所述细胞培养基质如果不是完全游离的，维生素D，雄激素激素，雄激素配体，雌激素激素，雌激素配体和/或雄激素受体基本上是游离的。