公开(公告)号：US20230287372A1

公开(公告)日：2023-09-14

申请号：US18006324

申请日：2021-07-20

Applicant: BRAIN Biotech AG

Inventor： Paul SCHOLZ ,  Christian ZUREK ,  Michael KROHN

IPC: C12N9/22 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/8201 ,  C12N2310/20

Abstract: The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 29, 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 30, 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 90%, preferably at least 92 %, and most preferably at least 95% identical to the amino acid sequence of (a); (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 90%, preferably at least 92%, and most preferably at least 95% identical to the nucleotide sequence of (b); (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.