公开(公告)号：US20080031858A1

公开(公告)日：2008-02-07

申请号：US11750863

申请日：2007-05-18

申请人： Barbara Chan ,  Godfrey Chan ,  Hoi Wong ,  Pik Cheung ,  Song-Eng Cheah ,  Danny Chan

发明人： Barbara Chan ,  Godfrey Chan ,  Hoi Wong ,  Pik Cheung ,  Song-Eng Cheah ,  Danny Chan

IPC分类号： A61K35/00 ,  A61P43/00 ,  C12M1/00 ,  C12N5/06 ,  C12P1/00

CPC分类号： C12N5/0602 ,  A61K35/12 ,  C12M25/14 ,  C12N5/0012 ,  C12N5/0655 ,  C12N5/0663 ,  C12N2510/02 ,  C12N2533/54 ,  C12N2533/90

摘要： A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.

摘要翻译： 已经开发了一种方法来生产具有高达100％包封效率和高细胞活力的稳定的细胞基质微球，使用具有差的形状和机械稳定性的基质或生物材料体系，包括通过显微注射或手术植入的细胞治疗剂，3D培养物 使用酶消化或机械解离并且用于增强治疗性生物分子的产生的体外扩增而不重复细胞分裂，以及用于形态发生研究的体外模拟。 改进的液滴生成方法简单可伸缩，当使用的基质或生物材料系统具有低浓度，缓慢的相变，形状和机械稳定性差时，能够生产细胞基质微球。