公开(公告)号：US20080031858A1

公开(公告)日：2008-02-07

申请号：US11750863

申请日：2007-05-18

申请人： Barbara Chan ,  Godfrey Chan ,  Hoi Wong ,  Pik Cheung ,  Song-Eng Cheah ,  Danny Chan

发明人： Barbara Chan ,  Godfrey Chan ,  Hoi Wong ,  Pik Cheung ,  Song-Eng Cheah ,  Danny Chan

IPC分类号： A61K35/00 ,  A61P43/00 ,  C12M1/00 ,  C12N5/06 ,  C12P1/00

CPC分类号： C12N5/0602 ,  A61K35/12 ,  C12M25/14 ,  C12N5/0012 ,  C12N5/0655 ,  C12N5/0663 ,  C12N2510/02 ,  C12N2533/54 ,  C12N2533/90

摘要： A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.

摘要翻译： 已经开发了一种方法来生产具有高达100％包封效率和高细胞活力的稳定的细胞基质微球，使用具有差的形状和机械稳定性的基质或生物材料体系，包括通过显微注射或手术植入的细胞治疗剂，3D培养物 使用酶消化或机械解离并且用于增强治疗性生物分子的产生的体外扩增而不重复细胞分裂，以及用于形态发生研究的体外模拟。 改进的液滴生成方法简单可伸缩，当使用的基质或生物材料系统具有低浓度，缓慢的相变，形状和机械稳定性差时，能够生产细胞基质微球。

公开(公告)号：US20070292514A1

公开(公告)日：2007-12-20

申请号：US11742040

申请日：2007-04-30

申请人： Barbara Chan Pui ,  Kenneth Cheung ,  Danny Chan ,  Godfrey Chan ,  Ting Hui

发明人： Barbara Chan Pui ,  Kenneth Cheung ,  Danny Chan ,  Godfrey Chan ,  Ting Hui

IPC分类号： A61F2/28 ,  A61K38/39 ,  A61P19/00 ,  C12N5/06 ,  C12N5/08

CPC分类号： A61F2/442 ,  A61F2/3094 ,  A61F2002/2817 ,  A61F2002/30006 ,  A61F2002/30016 ,  A61F2002/30153 ,  A61F2002/30677 ,  A61F2002/30971 ,  A61F2002/4445 ,  A61F2002/445 ,  A61F2230/0019 ,  A61F2250/0015 ,  A61F2250/0018 ,  A61F2250/0019 ,  A61F2310/00365 ,  A61K2035/128 ,  A61L27/3633 ,  A61L27/3834 ,  A61L27/3852 ,  A61L27/3856 ,  A61L2430/38 ,  C12N5/0663 ,  C12N2533/54

摘要： A bioengineered IVD for disc replacement has been developed that has mechanical and structural support characteristics similar to those of native IVD. Extracellular matrix (ECM) provides support to living cell components and interacts with the living cellular components during the fabrication process without introducing toxicity. The composition can be produced from both natural or synthetic source but preferably natural and induced to self-assemble or reconstitute into its solid form under conditions that are mild enough to support cellular survival and growth. The cells induce a volume change of the structures, leading to changes in dimension, ECM density, cell density mechanical property and stability, etc. The extent of the change i volume of the composition can be precisely controlled by factors such as the density of the ECM, the density of the living cells, the timing for interaction and the serum concentration. Increased structural support is provided by crosslinking.

摘要翻译： 已经开发了用于盘替代的生物工程IVD，其具有与天然IVD相似的机械和结构支持特征。 细胞外基质（ECM）在制造过程中提供对活细胞组分的支持和与活细胞组分的相互作用，而不引入毒性。 组合物可以天然或合成来源产生，但优选天然产生，并且在足够温和以支持细胞存活和生长的条件下诱导自组装或重组成其固体形式。 细胞诱导结构的体积变化，导致尺寸变化，ECM密度，细胞密度机械性能和稳定性等。组合物的体积变化程度可以由诸如 ECM，活细胞的密度，相互作用的时间和血清浓度。 通过交联提供增加的结构支撑。

公开(公告)号：USD494853S1

公开(公告)日：2004-08-24

申请号：US29195695

申请日：2003-12-16

申请人： Godfrey Chan

设计人： Godfrey Chan