公开(公告)号：US06171463B2

公开(公告)日：2001-01-09

申请号：US09301080

申请日：1999-04-28

申请人： Barton G. Selby ,  Johan Goudberg ,  Binh Vo ,  David Clark ,  Thomas Sch{grave over (e)}fer ,  Munechika Sakabe

发明人： Barton G. Selby ,  Johan Goudberg ,  Binh Vo ,  David Clark ,  Thomas Sch{grave over (e)}fer ,  Munechika Sakabe

IPC分类号： G01N2726

CPC分类号： G01N27/44743

摘要： The present invention relates to the electrophoretic separation of bio-organic molecules using a slab gel electrophoresis apparatus having a pair of spaced, confronting plates defining a multi-lane separation zone adapted to hold a separation medium and an upper loading zone. In one aspect of the invention, at least one of the plates is shaped to provide an expanded loading zone. The plate-to-plate distance within the expanded loading zone is greater than the plate-to-plate width within the separation zone. In another aspect of the invention, a comb is provided with each tooth having a gradual taper beginning at a support member and extending along most of the tooth's longitudinal axis, and a sharp taper beginning immediately beyond the gradual taper and extending to the end of the tooth. In a related aspect of the invention, a method is provided wherein a comb is inserted into the loading zone so that the teeth penetrate an upper portion of the separation medium, thereby forming a series of fluid-tight, elongate sample-loading apertures of substantially rectangular cross-section having a plate-to-plate width greater than that of the inter-plate distance in the separation zone. Methods of separating biomolecule analytes for optimized resolution and increased base reads are also disclosed.

摘要翻译： 本发明涉及使用板式凝胶电泳装置的生物有机分子的电泳分离，所述板式凝胶电泳装置具有限定适于保持分离介质和上装载区的多通道分离区的一对间隔开的面对板。 在本发明的一个方面中，至少一个板被成形为提供一个膨胀的加载区。 扩展加载区内的板间距离大于分离区内的板对板宽度。 在本发明的另一方面，梳子具有每个齿具有从支撑构件开始并且沿着该齿的纵向轴线的大部分延伸的渐变锥形的逐渐渐缩锥形，以及紧接着逐渐变细并且延伸至 齿。 在本发明的相关方面，提供了一种方法，其中将梳子插入装载区域中，使得齿穿透分离介质的上部，由此形成一系列流体密封的细长的样品加载孔，其基本上 矩形横截面的板对板宽度大于分离区中的板间距离。 还公开了用于优化分辨率和增加的碱基读数分离生物分子分析物的方法。

公开(公告)号：US5993628A

公开(公告)日：1999-11-30

申请号：US62381

申请日：1998-04-17

申请人： Barton G. Selby ,  Johan Goudberg ,  Binh Vo ,  David Clark

发明人： Barton G. Selby ,  Johan Goudberg ,  Binh Vo ,  David Clark

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44743

摘要： The present invention relates to the electrophoretic separation of bio-organic molecules using a slab gel electrophoresis apparatus having a pair of spaced, confronting plates defining a multi-lane separation zone adapted to hold a separation medium and an upper loading zone. In one aspect of the invention, at least one of the plates is shaped to provide an expanded loading zone. The plate-to-plate distance within the expanded loading zone is greater than the plate-to-plate width within the separation zone. In another aspect of the invention, a comb is provided with each tooth having a gradual taper beginning at a support member and extending along most of the tooth's longitudinal axis, and a sharp taper beginning immediately beyond the gradual taper and extending to the end of the tooth. In a related aspect of the invention, a method is provided wherein a comb is inserted into the loading zone so that the teeth penetrate an upper portion of the separation medium, thereby forming a series of fluid-tight, elongate sample-loading apertures of substantially rectangular cross-section having a plate-to-plate width greater than that of the inter-plate distance in the separation zone.

摘要翻译： 本发明涉及使用板式凝胶电泳装置的生物有机分子的电泳分离，所述板式凝胶电泳装置具有限定适于保持分离介质和上装载区的多通道分离区的一对间隔开的面对板。 在本发明的一个方面中，至少一个板被成形为提供一个膨胀的加载区。 扩展加载区内的板间距离大于分离区内的板对板宽度。 在本发明的另一方面，梳子具有每个齿具有从支撑构件开始并且沿着该齿的纵向轴线的大部分延伸的渐变锥形的逐渐渐缩锥形，以及紧接着逐渐变细并且延伸至 齿。 在本发明的相关方面，提供了一种方法，其中将梳子插入装载区域中，使得齿穿透分离介质的上部，由此形成一系列流体密封的细长的样品加载孔，其基本上 矩形横截面的板对板宽度大于分离区中的板间距离。

公开(公告)号：US20060070880A1

公开(公告)日：2006-04-06

申请号：US11215935

申请日：2005-08-31

申请人： Johan Goudberg ,  James Burrows

发明人： Johan Goudberg ,  James Burrows

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： C07K1/26 ,  G01N27/44704

摘要： Methods and apparatus for manipulating separation media in the context of filling one or more capillaries with a separation medium for electrophoresis. A polymer-displacement pump system and method for reciprocating a pump piston in a first direction to draw fresh fluid into a chamber, and reciprocating the pump piston in a second direction to cause the fresh fluid to exit the chamber and fill a single capillary or multi-capillary array. The pump piston movement can be electrically controlled.

摘要翻译： 在用电泳分离介质填充一个或多个毛细管的情况下操纵分离介质的方法和装置。 一种聚合物置换泵系统和方法，用于使泵活塞沿第一方向往复运动，以将新鲜流体吸入室中，并使泵活塞沿第二方向往复运动，以使新鲜流体离开室并填充单个毛细管或多个 毛细管阵列。 泵活塞运动可以电控制。