公开(公告)号：US20250052742A1

公开(公告)日：2025-02-13

申请号：US18709437

申请日：2021-11-12

Applicant: Beckman Coulter, Inc.

Inventor： Aditya JARARE ,  Rajesh VENKATESH ,  Sumeet CHAWLA ,  Mehul JIVRAJANI ,  Naina ARORA ,  Gopinadh JAKKA ,  Shiva Ranjini SRINIVASAN

IPC: G01N33/53 ,  C09B67/20 ,  C09B69/10 ,  C09K11/02 ,  C09K11/06 ,  G01N15/10 ,  G01N15/14 ,  G01N33/533

Abstract: A novel dry down buffer is provided for use in drying a plurality of fluorescent dye conjugates on a substrate for use in flow cytometry. The aqueous buffer comprises a water-soluble monomer; a protein stabilizer; a carbohydrate stabilizer; and a zwitterionic surfactant. When mixed with a multi-color panel comprising fluorescent polymer dye conjugates, dried on a substrate, and reconstituted with a biological sample, the buffer provides decreased aggregation of fluorescent polymer dye conjugates, and decreased non-specific binding of monocytes and granulocytes, when compared to use of a buffer without the water-soluble monomer or zwitterionic surfactant.