公开(公告)号：US20230295609A1

公开(公告)日：2023-09-21

申请号：US18179144

申请日：2023-03-06

Applicant: Becton, Dickinson And Company

Inventor： Jing Hu ,  Christina Fan ,  David Rosenfeld ,  Jue Fan ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  G16B20/00 ,  G16B45/00 ,  C12Q1/6869

CPC classification number: C12N15/1065 ,  G16B20/00 ,  G16B45/00 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2535/122

Abstract: Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.

公开(公告)号：US11608497B2

公开(公告)日：2023-03-21

申请号：US15806174

申请日：2017-11-07

Applicant: Becton, Dickinson and Company

Inventor： Jing Hu ,  Christina Fan ,  David Rosenfeld ,  Jue Fan ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  G16B45/00 ,  C12Q1/6869 ,  G16B20/00

Abstract: Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.

公开(公告)号：US11164659B2

公开(公告)日：2021-11-02

申请号：US15806223

申请日：2017-11-07

Applicant: Becton, Dickinson and Company

Inventor： Jue Fan ,  Jesse Zhang ,  Jing Hu

IPC: G16B40/30 ,  G06F16/28 ,  G06F16/22 ,  G16B40/00 ,  G16B25/00 ,  G16B99/00 ,  G16B25/10

Abstract: Disclosed herein are methods and systems for identifying targets for distinguishing cell types. In some embodiments, the method comprises: hierarchically clustering expression profiles of cells to generate a dendrogram with each leaf representing the expression profile of a different individual cell. The dendrogram can be pruned by eliminating invalid splits (and their children). The remaining leaves in the dendrogram can be merged, independent of their locations in the dendrogram, based on their distances to one another to generate clusters of expression profiles. The method can identify targets for distinguishing cell types based on targets that are expressed differentially in the clusters.