公开(公告)号：US20070009572A1

公开(公告)日：2007-01-11

申请号：US11176654

申请日：2005-07-06

申请人： Bee Mary Chan ,  Jinye Shen ,  Bin He ,  Aiping Zhu ,  Yehai Yan ,  Chee Yue

发明人： Bee Mary Chan ,  Jinye Shen ,  Bin He ,  Aiping Zhu ,  Yehai Yan ,  Chee Yue

IPC分类号： A61F2/02 ,  B29C45/00

CPC分类号： B29C33/3857 ,  B29C33/424

摘要： In embodiments of the present invention, a biodegradable/biodegradable polymer film may be used as a scaffold for tissue engineering scaffolds for engineering organized organs, such as vascular grafts, for example. In one embodiment, an ultraviolet (UV) resin made from a diacrylated biodegradable oligomer is molded into a flexible scaffold having cavities and/or channels. Channel/cavity size may be on the order of micrometers and/or nanometers, and thus the walls may have high aspect ratios. Smooth muscle cells may be deposited in the channels and because of the high aspect ratios, the cells may align along the channels/cavities as confluence is reached.

摘要翻译： 在本发明的实施方案中，可生物降解/可生物降解的聚合物膜可用作用于组织工程支架的支架，用于工程组织的器官，例如血管移植物。 在一个实施方案中，由二丙烯酸酯化的可生物降解的低聚物制成的紫外线（UV）树脂被模制成具有空腔和/或通道的柔性支架。 通道/腔尺寸可以在微米级和/或纳米级上，因此壁可具有高纵横比。 平滑肌细胞可以沉积在通道中，并且由于高纵横比，当达到汇合时，细胞可以沿着通道/腔排列。

公开(公告)号：US08906282B2

公开(公告)日：2014-12-09

申请号：US11176654

申请日：2005-07-06

申请人： Bee Eng Mary Chan ,  Jinye Shen ,  Bin He ,  Aiping Zhu ,  Yehai Yan ,  Chee Yoon Yue

发明人： Bee Eng Mary Chan ,  Jinye Shen ,  Bin He ,  Aiping Zhu ,  Yehai Yan ,  Chee Yoon Yue

IPC分类号： B27N3/08 ,  B29C41/00 ,  B28B7/30 ,  A61F2/00 ,  A61K9/14 ,  B29C33/38 ,  B29C33/42

CPC分类号： B29C33/3857 ,  B29C33/424

摘要： In embodiments of the present invention, a biodegradable/biodegradable polymer film may be used as a scaffold for tissue engineering scaffolds for engineering organized organs, such as vascular grafts, for example. In one embodiment, an ultraviolet (UV) resin made from a diacrylated biodegradable oligomer is molded into a flexible scaffold having cavities and/or channels. Channel/cavity size may be on the order of micrometers and/or nanometers, and thus the walls may have high aspect ratios. Smooth muscle cells may be deposited in the channels and because of the high aspect ratios, the cells may align along the channels/cavities as confluence is reached.

摘要翻译： 在本发明的实施方案中，可生物降解/可生物降解的聚合物膜可用作用于组织工程支架的支架，用于工程组织的器官，例如血管移植物。 在一个实施方案中，由二丙烯酸酯化的可生物降解的低聚物制成的紫外线（UV）树脂被模制成具有空腔和/或通道的柔性支架。 通道/腔尺寸可以在微米级和/或纳米级上，因此壁可具有高纵横比。 平滑肌细胞可以沉积在通道中，并且由于高纵横比，当达到汇合时，细胞可以沿着通道/腔排列。

公开(公告)号：US20090248145A1

公开(公告)日：2009-10-01

申请号：US12058580

申请日：2008-03-28

申请人： Bee Eng Chan ,  Jie Feng ,  Jinye Shen ,  Vincent Chan

发明人： Bee Eng Chan ,  Jie Feng ,  Jinye Shen ,  Vincent Chan

IPC分类号： A61F2/06 ,  C12N5/06

CPC分类号： C12N5/0691 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/507 ,  C12N2535/10

摘要： The present invention provides a method of forming a three-dimensional structure of unidirectionally aligned cells. The method comprises providing a substrate with a microchannel. The microchannel is defined by at least a pair of opposing lateral walls and a base. In at least a portion of the microchannel the distance between the pair of opposing lateral walls is within the micrometer range. A first plurality of cells is seeded in the microchannel and the cells are allowed to proliferate up to at least a density of at least 90%. Thereby contact guidance cues are provided by the pair of opposing lateral walls of the microchannel, such that the cells align unidirectionally. Thereby a first layer of aligned cells is also formed at the base of the microchannel. A second plurality of cells is seeded in the microchannel, which already comprises a first layer of aligned cells. When allowing cells of the second plurality of cells to proliferate up to at least substantial confluence, contact guidance cues are again provided by the lateral walls of the microchannel. The cells also align unidirectionally and form a second layer of aligned cells.

摘要翻译： 本发明提供了形成单向排列单元的三维结构的方法。 该方法包括向基底提供微通道。 微通道由至少一对相对的侧壁和底座限定。 在微通道的至少一部分中，一对相对的侧壁之间的距离在微米范围内。 将第一组多个细胞接种在微通道中，并使细胞增殖至少至少90％的密度。 因此，接触引导线索由微通道的一对相对的侧壁提供，使得单元单向对准。 由此，在微通道的基部也形成第一层对准的单元。 将第二组多个单元接种在微通道中，该微通道已经包括第一层对准的单元。 当允许第二多个细胞的细胞增殖至少至少基本汇合时，接触引导线索再次由微通道的侧壁提供。 单元还单向对准并形成第二层对准的单元。