公开(公告)号：US20140088180A1

公开(公告)日：2014-03-27

申请号：US14093078

申请日：2013-11-29

Applicant: Beijing Bio-Targeting Therapeutics Technology Inc.

Inventor： Yaohe WANG ,  Guozhong JIANG ,  Hanshi WONG ,  Fengyu CAO ,  Nick LEMOINE

IPC: C12N15/86

CPC classification number: C12N15/86 ,  A61K35/761 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343

Abstract: A method for constructing a subgroup B recombinant human adenovirus vector Ad11-5EP. The method includes substituting a 365 bp fragment including an enhancer and a promoter of an upstream coding sequence of Ad5 E1A for a corresponding region of a serotype Ad11 of the subgroup B human adenovirus vector by homologous recombination to construct the subgroup B recombinant human adenovirus vector Ad11-5EP. A subgroup B recombinant human adenovirus vector Ad11-5EP constructed by the method and the use thereof for treatment of tumors are also provided.

Abstract translation: 一种构建亚组B重组人腺病毒载体Ad11-5EP的方法。 该方法包括通过同源重组将包含增强子的365bp片段和Ad5 E1A上游编码序列的启动子替换为亚组B人腺病毒载体的血清型Ad11的相应区域，以构建亚组B重组人腺病毒载体Ad11 -5EP。 还提供了通过该方法构建的亚组B重组人腺病毒载体Ad11-5EP及其用于治疗肿瘤的用途。

公开(公告)号：US20180187214A1

公开(公告)日：2018-07-05

申请号：US15904408

申请日：2018-02-25

Applicant: Beijing Bio-Targeting Therapeutics Technology Inc.

Inventor： Yaohe WANG ,  Guozhong JIANG ,  Hanshi WONG ,  Fengyu CAO ,  Nick LEMOINE

IPC: C12N15/86 ,  A61K35/761 ,  C12N7/00

CPC classification number: C12N15/86 ,  A61K35/761 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343

Abstract: A subgroup B recombinant human adenovirus vector Ad11-5EP (SEQ ID NO: 1), which is constructed by a method including: substituting a 365 bp fragment containing an enhancer and a promoter of an upstream coding sequence of Ad5 E1A (SEQ ID NO: 2) for a corresponding region of a serotype Ad11 (SEQ ID NO: 3) of a subgroup B human adenovirus vector by homologous recombination to construct the subgroup B recombinant human adenovirus vector Ad11-5EP (SEQ ID NO: 1).

公开(公告)号：US20160222413A1

公开(公告)日：2016-08-04

申请号：US15098342

申请日：2016-04-14

Applicant: Beijing Bio-Targeting Therapeutics Technology Inc.

Inventor： Yaohe WANG ,  Guozhong JIANG ,  Hanshi WONG ,  Fengyu CAO ,  Nick LEMOINE

IPC: C12N15/86 ,  C12N7/00

CPC classification number: C12N15/86 ,  A61K35/761 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343

Abstract: A method for reconstructing a replication-selective oncolytic adenovirus using a subgroup B recombinant human adenovirus vector Ad11-5EP. The method includes: 1) deleting E1A CR2 gene and/or anti-apoptotic gene E1B 21K that is necessary for viability of an adenovirus in normal cells but not necessary in tumor cells; 2) inserting a tumor-specific promoter to drive the expression of E1A gene; 3) re-directing a cellular tropism of Ad11-5EP according to receptors on a tumor cell surface; or 4) allowing adenovirus to selectively replicate in tumor cells.

Abstract translation: 使用亚组B重组人腺病毒载体Ad11-5EP重建复制选择性溶瘤腺病毒的方法。 该方法包括：1）删除正常细胞中腺病毒存活所必需的E1A CR2基因和/或抗凋亡基因E1B 21K，但在肿瘤细胞中不是必需的; 2）插入肿瘤特异性启动子以驱动E1A基因的表达; 3）根据肿瘤细胞表面上的受体重新导向Ad11-5EP的细胞向性; 或4）允许腺病毒在肿瘤细胞中选择性复制。