Quasi-interpenetrating networks used as separation media
    1.
    发明授权
    Quasi-interpenetrating networks used as separation media 失效
    准互穿网络用作分离介质

    公开(公告)号:US07037978B2

    公开(公告)日:2006-05-02

    申请号:US10671335

    申请日:2003-09-25

    IPC分类号: C08L9/02 C08L33/20

    摘要: The invention relates to a quasi-interpenetrating network of polymer chains, the chains comprising linear polyacrylamide (LPA) chains in the form of a main frame having a weight average molecular weight of approximately 0.05 million to approximately 25 million g/mole, and a radius of gyration of approximately 10 nm to 350 nm; and polydimethylacrylamide (PDMA) chains prepared by polymerizing PDMA in the presence of the LPA main frame, wherein the LPA and PDMA chains are entangled within one another and interpenetrate one another, and wherein the quasi-interpenetrating network has substantially no chemical cross-linking.

    摘要翻译: 本发明涉及一种聚合物链的准互穿网络,该链包括主框形式的线性聚丙烯酰胺(LPA)链,其重均分子量约为0.05〜2500万g / mol,半径 的旋转约10nm至350nm; 和通过在LPA主链的存在下聚合PDMA而制备的聚二甲基丙烯酰胺(PDMA)链,其中LPA和PDMA链彼此缠结并相互渗透,并且其中准互穿网络基本上没有化学交联。

    Quasi-interpenetrating networks used as separation media
    2.
    发明申请
    Quasi-interpenetrating networks used as separation media 失效
    准互穿网络用作分离介质

    公开(公告)号:US20050070668A1

    公开(公告)日:2005-03-31

    申请号:US10671335

    申请日:2003-09-25

    IPC分类号: C08L33/14 C08L39/00

    摘要: The invention relates to a quasi-interpenetrating network of polymer chains, the chains comprising linear polyacrylamide (LPA) chains in the form of a main frame having a weight average molecular weight of approximately 0.05 million to approximately 25 million g/mole, and a radius of gyration of approximately 10 nm to 350 nm; and polydimethylacrylamide (PDMA) chains prepared by polymerizing PDMA in the presence of the LPA main frame, wherein the LPA and PDMA chains are entangled within one another and interpenetrate one another, and wherein the quasi-interpenetrating network has substantially no chemical cross-linking.

    摘要翻译: 本发明涉及一种聚合物链的准互穿网络,该链包括主框形式的线性聚丙烯酰胺(LPA)链,其重均分子量约为0.05〜2500万g / mol,半径 的旋转约10nm至350nm; 和通过在LPA主链的存在下聚合PDMA而制备的聚二甲基丙烯酰胺(PDMA)链,其中LPA和PDMA链彼此缠结并相互渗透,并且其中准互穿网络基本上没有化学交联。

    Polymer solution for separation of charged macromolecules by electrophoresis
    3.
    发明授权
    Polymer solution for separation of charged macromolecules by electrophoresis 失效
    用于通过电泳分离带电荷大分子的聚合物溶液

    公开(公告)号:US06770698B1

    公开(公告)日:2004-08-03

    申请号:US09586628

    申请日:2000-06-05

    IPC分类号: C08L5100

    摘要: The present invention is a polymer solution for the efficient separation of charged macromolecules by electrophoresis that includes a plurality of polymers. These polymers are entangled to form an interpenetrating network with greater entanglement times than corresponding homopolymers of the same length. These polymers are different and do not phase separate when dissolved in solution. Preferred polymers are PAM and PVP or PDAM and PVP. The polymer solutions of the present invention provide at least a 500-base read length in one run for a single-stranded DNA separation.

    摘要翻译: 本发明是一种聚合物溶液,用于通过包括多种聚合物的电泳有效分离带电荷的大分子。 这些聚合物缠结以形成具有相同长度的相应均聚物的缠结时间的互穿网络。 这些聚合物是不同的,当溶于溶液时不分相。 优选的聚合物是PAM和PVP或PDAM和PVP。 本发明的聚合物溶液在一次运行中提供至少500个碱基读取长度用于单链DNA分离。