公开(公告)号：US20050102712A1

公开(公告)日：2005-05-12

申请号：US10637763

申请日：2003-08-08

申请人： Bernhard Grimm ,  Jutta Papenbrock ,  Frank Hanel ,  Susanna Grafe ,  Frank Schmidt ,  Wolfgang Streber

发明人： Bernhard Grimm ,  Jutta Papenbrock ,  Frank Hanel ,  Susanna Grafe ,  Frank Schmidt ,  Wolfgang Streber

IPC分类号： A01H5/00 ,  C07K16/16 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N15/09 ,  C12N15/82 ,  C12Q1/68 ,  A01H1/00 ,  C12Q1/48

CPC分类号： C12N9/00 ,  C12N15/8269 ,  C12N15/8274

摘要： The present invention relates to a nucleic acid molecule which encodes a protein with the function of a plant Mg chelatase subunit CHLD or an active fragment thereof; a protein which has the function of a plant Mg chelatase subunit CHLD or an active fragment thereof, preferably a recombinant protein; a method of determining the interaction of plant Mg chelatase subunits, in which a host cell is transformed with a DNA sequence as claimed in one or more of claims 1 to 3 and at least with one DNA sequence encoding a further subunit of Mg chelatase in such a manner that the interaction of the Mg chelatase gene products leads to a directly or indirectly, qualitatively or quantitatively measurable signal, preferably by activating a marker gene, and transgenic plants, transgenic plant cells, transgenic plant organs, transgenic plant seeds, transgenic propagation material comprising an abovementioned nucleic acid molecule.

摘要翻译： 本发明涉及编码具有植物Mg螯合酶亚基CHLD或其活性片段的功能的蛋白质的核酸分子; 具有植物Mg螯合酶亚单位CHLD或其活性片段，优选重组蛋白质的功能的蛋白质; 确定植物Mg螯合酶亚基的相互作用的方法，其中宿主细胞用如权利要求1至3中的一项或多项所述的DNA序列转化，并且至少编码一种编码其中的Mg螯合酶亚单位的DNA序列 Mg螯合酶基因产物的相互作用导致直接或间接，定性或定量可信号的信号，优选通过激活标记基因和转基因植物，转基因植物细胞，转基因植物器官，转基因植物种子，转基因繁殖材料 包含上述核酸分子。