公开(公告)号：US07119898B2

公开(公告)日：2006-10-10

申请号：US10763649

申请日：2004-01-23

申请人： Bernhard Zimmermann ,  Eva Simbuerger ,  Mary Dickinson

发明人： Bernhard Zimmermann ,  Eva Simbuerger ,  Mary Dickinson

IPC分类号： G01J3/30

CPC分类号： G02B21/008 ,  G01N21/64 ,  G01N2021/6419 ,  G01N2021/6441 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/16

摘要： A method for the detection and evaluation of the light generated in a fluorescing specimen by a short pulse laser, wherein at least a first and a second fluorophore and/or a self-fluorescing specimen are separately irradiated with different wavelengths and the specimen light is recorded in a wavelength-dependent manner with at least one nondescanned detector as reference spectrum and a separation into individual spectra is carried out during the irradiation of at least two fluorophores and/or self-fluorescing specimens simultaneously from the measured spectrum and the reference spectra through regression analysis, wherein the wavelength of the short pulse laser is advantageously changed continuously in at least one wavelength region.

摘要翻译： 一种用于通过短脉冲激光检测和评估在荧光样品中产生的光的方法，其中至少第一和第二荧光团和/或自发荧光样品被不同波长分开照射并且记录样本光 以至少一个非扫描检测器作为参考光谱的波长依赖性方式，并且在从测量的光谱和参考光谱通过回归同时从至少两个荧光团和/或自发荧光样品的照射期间进行分离成单个光谱 分析，其中短脉冲激光器的波长有利地在至少一个波长区域中连续地改变。

公开(公告)号：US20140138517A1

公开(公告)日：2014-05-22

申请号：US14127067

申请日：2012-06-15

申请人： Mirko Liedtke ,  Eva Simbuerger ,  Daniel Schwedt

发明人： Mirko Liedtke ,  Eva Simbuerger ,  Daniel Schwedt

IPC分类号： G02B21/00

CPC分类号： G02B21/008 ,  G02B21/0076 ,  H01J43/30

摘要： A switch which reduces the voltage between the photocathode and the first dynode in the activated switching state compared to the deactivated switching state and a control unit which is adapted to move a target spot, which can be illuminated by means of the light source, over a scanning field by means of a deflecting unit. The control unit activates the switch when the target spot enters a given region of the scanning field and deactivates the switch when the target spot exits the region.

摘要翻译： 一种开关，其与处于去激活的开关状态相比，在激活的开关状态下降低光电阴极和第一倍增电极之间的电压;以及控制单元，其适于移动可以通过光源照亮的目标点， 通过偏转单元进行扫描场。 当目标点进入扫描场的给定区域时，控制单元激活开关，当目标点离开区域时，控制单元停止开关。

公开(公告)号：US09927603B2

公开(公告)日：2018-03-27

申请号：US14127067

申请日：2012-06-15

申请人： Mirko Liedtke ,  Eva Simbuerger ,  Daniel Schwedt

发明人： Mirko Liedtke ,  Eva Simbuerger ,  Daniel Schwedt

IPC分类号： G02B21/00 ,  H01J43/30

CPC分类号： G02B21/008 ,  G02B21/0076 ,  H01J43/30

摘要： A switch which reduces the voltage between the photocathode and the first dynode in the activated switching state compared to the deactivated switching state and a control unit which is adapted to move a target spot, which can be illuminated by means of the light source, over a scanning field by means of a deflecting unit. The control unit activates the switch when the target spot enters a given region of the scanning field and deactivates the switch when the target spot exits the region.