公开(公告)号：US20060255237A1

公开(公告)日：2006-11-16

申请号：US11487936

申请日：2006-07-17

申请人： Thomas Mehner ,  Stefan Wilhelm ,  Ulrich Meisel ,  Mirko Liedtke

发明人： Thomas Mehner ,  Stefan Wilhelm ,  Ulrich Meisel ,  Mirko Liedtke

IPC分类号： G02B27/40

CPC分类号： G02B21/0032 ,  G02B21/008

摘要： An arrangement for the detection of the illumination radiation in a laser scanning microscope, wherein a portion of the illumination radiation is coupled out and detected at the main color splitter, wherein light transmitted through the main color splitter is advantageously detected and/or a portion of the illumination radiation is coupled out and detected before coupling into a light-conducting fiber and/or a portion of the illumination radiation is coupled out and detected at a beam splitter arranged downstream of a light-conducting fiber.

公开(公告)号：US20050035281A1

公开(公告)日：2005-02-17

申请号：US10888260

申请日：2004-07-09

申请人： Thomas Mehner ,  Stefan Wilhelm ,  Ulrich Meisel ,  Mirko Liedtke

发明人： Thomas Mehner ,  Stefan Wilhelm ,  Ulrich Meisel ,  Mirko Liedtke

IPC分类号： G02B21/06 ,  G02B21/00 ,  H01J3/14 ,  G02F1/01 ,  H01J5/16

CPC分类号： G02B21/0032 ,  G02B21/008

摘要： An arrangement for the detection of the illumination radiation in a laser scanning microscope, wherein a portion of the illumination radiation is coupled out and detected at the main color splitter, wherein light transmitted through the main color splitter is advantageously detected and/or a portion of the illumination radiation is coupled out and detected before coupling into a light-conducting fiber and/or a portion of the illumination radiation is coupled out and detected at a beam splitter arranged downstream of a light-conducting fiber.

摘要翻译： 一种用于在激光扫描显微镜中检测照射辐射的装置，其中照射辐射的一部分耦合到主分色器并在主分色器处被检测，其中有利地检测透过主分色器的光和/或部分 照射辐射在耦合到导光纤维之前被耦合出来并被检测，并且/或者一部分照射辐射被耦合出来，并在布置在导光纤维下游的分束器处被检测。

公开(公告)号：US20120162754A1

公开(公告)日：2012-06-28

申请号：US13334195

申请日：2011-12-22

申请人： Mirko LIEDTKE

发明人： Mirko LIEDTKE

IPC分类号： G02B21/06

CPC分类号： G02B21/006 ,  G02B21/008

摘要： A laser scanning microscope comprising having a light source, an illuminating beam for illuminating a specimen with a spatially limited, preferably point-shaped, illuminating light spot via a scanning device and an objective lens, and a detection beam for detecting the specimen light that reaches the detection beam via the objective lens and the scanning device. Focusing means that are disposed in the detection beam and focus the light from the specimen to form a spatially limited detection light spot in a plane. Receiver elements are distributed in the spot in a matrix-like manner and can be individually read out. These are located in order to simulate an adjustable pinhole aperture.

摘要翻译： 一种激光扫描显微镜，包括：具有光源的光源，用于通过扫描装置和物镜用空间有限的优选点状的照明光点照射样本的照明光束，以及用于检测到达到的样本光的检测光束 通过物镜和扫描装置的检测光束。 聚焦装置设置在检测光束中并聚焦来自样品的光，以在平面中形成空间有限的检测光斑。 接收器元件以矩阵状的方式分布在现场，并可以单独读出。 这些位置是为了模拟可调针孔。

公开(公告)号：US07999238B2

公开(公告)日：2011-08-16

申请号：US12375389

申请日：2007-07-16

申请人： Ralf Wolleschensky ,  Mirko Liedtke

发明人： Ralf Wolleschensky ,  Mirko Liedtke

IPC分类号： G01N21/64

CPC分类号： G02B21/0084 ,  G01N21/6408 ,  G02B21/008

摘要： An arrangement for signal processing at the output of a multichannel detector in the spectrally resolved acquisition of time-variable fluorescence phenomena in a microscope, particularly lifetime measurements, is characterized in that an FPGA (free programmable gate array) is arranged downstream of the output of the multichannel detector.

摘要翻译： 在显微镜中对时变荧光现象进行光谱分辨采集，特别是寿命测量的多通道检测器的输出处的信号处理装置的特征在于，FPGA（自由可编程门阵列）布置在 多通道检测器。

公开(公告)号：US20110119034A1

公开(公告)日：2011-05-19

申请号：US12970019

申请日：2010-12-16

申请人： Mirko LIEDTKE ,  Frank KLEMM ,  Manfred LOTH

发明人： Mirko LIEDTKE ,  Frank KLEMM ,  Manfred LOTH

IPC分类号： G06F15/00

CPC分类号： G02B21/0076 ,  G01J3/2889 ,  G01J3/4406

摘要： A method for recording pulse signals which allows the reconstruction of a time reference. The time of every pulse signal event can be determined by counting sampling result bits preceding the respective sampling result bit using the known sampling frequency. For this purpose, every period of the sampling frequency is associated with a bit representing the respective sampling result and the sampling result bits are stored one by one and per channel in data blocks. The sampling frequency is preferably higher than a pixel clock, a sampling result bit associated with a flank of the pixel clock being marked. The pixel clock can thus be synchronized with the individual events exactly per sampling period. The invention further relates to the field of fluorescence correlation spectroscopy using confocal microscopes or laser scanning microscopes.

摘要翻译： 一种用于记录允许重建时间基准的脉冲信号的方法。 可以通过使用已知的采样频率对相应采样结果位之前的采样结果位进行计数来确定每个脉冲信号事件的时间。 为此，采样频率的每个周期都与表示相应采样结果的位相关联，采样结果位逐个存储，每个通道存储在数据块中。 采样频率优选地高于像素时钟，与被标记的像素时钟的侧面相关联的采样结果位。 因此，像素时钟可以在每个采样周期精确地与各个事件同步。 本发明还涉及使用共焦显微镜或激光扫描显微镜的荧光相关光谱领域。

公开(公告)号：US07869123B2

公开(公告)日：2011-01-11

申请号：US11806458

申请日：2007-05-31

申请人： Gunter Moehler ,  Mirko Liedtke

发明人： Gunter Moehler ,  Mirko Liedtke

IPC分类号： G02B21/00

CPC分类号： H04L12/407 ,  G02B21/008 ,  G02B21/365 ,  H04L2012/40215

摘要： Specimen laser-scanning microscope with raster scanning illumination and detector modules, which illuminates and detects a specimen by raster scanning. A real-time control device (device) performs synchronous reading-out with the raster scanning pixel cycle. A data port serially communicates with the device using a bidirectional high-speed data stream and with the resources via a serial, bidirectional high-speed data stream with a data conversion to/from parallel to serial. The high-speed data stream is made up of data packets with data bits and type bits and no additional header or protocol bits. The data bits contain data from/on the resources and the type bits code the type of data. Type information is stored in the resources as well as the device. The type information defines processing functions for data types coded by the type bits, and the resources and/or the device determine the data type using type bits and process data coded in the data bits.

摘要翻译： 具有光栅扫描照明和检测器模块的样品激光扫描显微镜，其通过光栅扫描照亮和检测样品。 实时控制装置（装置）利用光栅扫描像素周期执行同步读出。 数据端口使用双向高速数据流和通过串行双向高速数据流与资源串行地与设备进行通信，数据转换到并行到串行数据转换。 高速数据流由具有数据位和类型位的数据分组组成，没有附加的报头或协议位。 数据位包含来自资源的数据，类型位代码数据类型。 类型信息存储在资源以及设备中。 类型信息定义由类型位编码的数据类型的处理功能，并且资源和/或设备使用在数据位中编码的类型位和处理数据来确定数据类型。

公开(公告)号：US20090250627A1

公开(公告)日：2009-10-08

申请号：US12375389

申请日：2007-07-16

申请人： Ralf Wolleschensky ,  Mirko Liedtke

发明人： Ralf Wolleschensky ,  Mirko Liedtke

IPC分类号： G01N21/64

CPC分类号： G02B21/0084 ,  G01N21/6408 ,  G02B21/008

摘要： An arrangement for signal processing at the output of a multichannel detector in the spectrally resolved acquisition of time-variable fluorescence phenomena in a microscope, particularly lifetime measurements, is characterized in that an FPGA (free programmable gate array) is arranged downstream of the output of the multichannel detector.

摘要翻译： 在显微镜中对时变荧光现象进行光谱分辨采集，特别是寿命测量的多通道检测器的输出处的信号处理装置的特征在于，FPGA（自由可编程门阵列）布置在 多通道检测器。

公开(公告)号：US20140138517A1

公开(公告)日：2014-05-22

申请号：US14127067

申请日：2012-06-15

申请人： Mirko Liedtke ,  Eva Simbuerger ,  Daniel Schwedt

发明人： Mirko Liedtke ,  Eva Simbuerger ,  Daniel Schwedt

IPC分类号： G02B21/00

CPC分类号： G02B21/008 ,  G02B21/0076 ,  H01J43/30

摘要： A switch which reduces the voltage between the photocathode and the first dynode in the activated switching state compared to the deactivated switching state and a control unit which is adapted to move a target spot, which can be illuminated by means of the light source, over a scanning field by means of a deflecting unit. The control unit activates the switch when the target spot enters a given region of the scanning field and deactivates the switch when the target spot exits the region.

摘要翻译： 一种开关，其与处于去激活的开关状态相比，在激活的开关状态下降低光电阴极和第一倍增电极之间的电压;以及控制单元，其适于移动可以通过光源照亮的目标点， 通过偏转单元进行扫描场。 当目标点进入扫描场的给定区域时，控制单元激活开关，当目标点离开区域时，控制单元停止开关。

公开(公告)号：US08497464B2

公开(公告)日：2013-07-30

申请号：US12856792

申请日：2010-08-16

申请人： Wolfgang Bathe ,  Michael Goelles ,  Mirko Liedtke

发明人： Wolfgang Bathe ,  Michael Goelles ,  Mirko Liedtke

IPC分类号： G01D5/30 ,  H01J3/14 ,  H01L27/00

CPC分类号： G01J1/04 ,  G01J1/02 ,  G01J1/0209 ,  G01J1/0407 ,  G01J1/0411 ,  G01J1/0422 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/0208 ,  G01J3/0216 ,  G01J3/0256 ,  G02B5/045 ,  G02B21/0064 ,  G02B21/008 ,  H01L27/14625 ,  H01L27/14806 ,  H01L31/02325

摘要： To increase the sensitivity of detector arrangements, it is known that light deflection elements in the form of a line arrays having spherical elements may be used to focus incident light onto light-sensitive regions of the detector. Manufacturing such line arrays is complex and cost intensive, especially in small lot numbers. The increased sensitivity of the detector array can be achieved easily and inexpensively by using a novel light deflection element. The detector arrangement therefore has a light deflection element having light entrance surfaces, deflecting incident light by refraction onto light-sensitive regions of the detector. Light entrance surfaces of the light deflection element are inclined with respect to one another and are designed as planar surfaces. The detector arrangement is suitable in particular for detection of light emanating from a specimen in a microscope, preferably in a laser-scanning microscope.

摘要翻译： 为了提高检测器装置的灵敏度，已知可以使用具有球形元件的线阵列形式的光偏转元件将入射光聚焦到检测器的光敏区域上。 制造这样的线阵列是复杂和成本密集的，特别是在很少的批号。 通过使用新颖的光偏转元件，可以容易且廉价地实现检测器阵列的增加的灵敏度。 因此，检测器装置具有具有光入射表面的光偏转元件，通过折射将入射光偏转到检测器的光敏区域上。 光偏转元件的光入射面相对于彼此倾斜并被设计为平面。 检测器装置特别适于检测在显微镜中优选在激光扫描显微镜中从样品发出的光。

公开(公告)号：US07859673B2

公开(公告)日：2010-12-28

申请号：US11822152

申请日：2007-07-02

申请人： Gunter Moehler ,  Dietmar Schmidt ,  Mirko Liedtke

发明人： Gunter Moehler ,  Dietmar Schmidt ,  Mirko Liedtke

IPC分类号： G01N21/55 ,  H03K19/14

CPC分类号： G01J1/44 ,  G01J1/42 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16

摘要： A method for detecting and analyzing light signals, in which a light signal impinges an optoelectric converter, where it is converted into an electric signal and the electric signal subsequent to the conversion is distributed into several analysis channels (13.1-13.4), within each analysis channel (13.1), (i) a signal analysis is performed, which is different from the signal analysis for the other analysis channels (13.2-13.4) and (ii) an output signal is created. In such a method, one or more output signals are selected for further processing and output using a specified, changeable selection criterion.

摘要翻译： 一种用于检测和分析其中光信号照射光电转换器的光信号的方法，其中它被转换为电信号，并且在转换之后的电信号被分配到多个分析通道（13.1-13.4）中，在每个分析 通道（13.1），（i）进行与其他分析通道（13.2-13.4）的信号分析不同的信号分析，（ii）产生输出信号。 在这种方法中，选择一个或多个输出信号用于使用指定的可变选择标准进一步处理和输出。