公开(公告)号：US06913908B2

公开(公告)日：2005-07-05

申请号：US09935757

申请日：2001-08-24

申请人： Bettina Möckel ,  Thoams Hermann ,  Mike Farwick ,  Michael Binder ,  Walter Pfefferle

发明人： Bettina Möckel ,  Thoams Hermann ,  Mike Farwick ,  Michael Binder ,  Walter Pfefferle

IPC分类号： C07K14/34 ,  C12P13/08 ,  C12P13/04

CPC分类号： C12R1/15 ,  C07K14/34 ,  C12P13/08

摘要： The present invention relates lo an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group c insisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of(a) or (b), and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.

摘要翻译： 本发明涉及一种来自谷氨酸棒杆菌的分离的多核苷酸，其包含选自下组的多核苷酸序列，所述多核苷酸序列坚持：（a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含氨基 SEQ ID NO：2的酸序列; （b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％的程度相同的氨基酸序列; （c）与（a）或（b）的多核苷酸互补的多核苷酸，和（d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸， 以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sigE基因以增强形式存在，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

公开(公告)号：US20050221450A1

公开(公告)日：2005-10-06

申请号：US11137504

申请日：2005-05-26

申请人： Bettina Mockel ,  Thoams Hermann ,  Mike Farwick ,  Michael Binder ,  Walter Pfefferle

发明人： Bettina Mockel ,  Thoams Hermann ,  Mike Farwick ,  Michael Binder ,  Walter Pfefferle

IPC分类号： C07K14/34 ,  C12P13/08 ,  C12P13/04 ,  C07H21/04 ,  C12N9/10 ,  C12N9/12 ,  C12N15/74

CPC分类号： C12R1/15 ,  C07K14/34 ,  C12P13/08

摘要： The present invention relates to an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group consisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b); and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.

摘要翻译： 本发明涉及来自谷氨酸棒杆菌的分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自（a）与编码包含氨基酸的多肽的多核苷酸的至少70％的程度相同的多核苷酸 SEQ ID NO：2的序列; （b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％的程度相同的氨基酸序列; （c）与（a）或（b）的多核苷酸互补的多核苷酸; 和（d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少 sigE基因以增强形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。