公开(公告)号：US20020182717A1

公开(公告)日：2002-12-05

申请号：US10098739

申请日：2002-03-14

申请人： Biacore AB

发明人： Olof Karlsson

IPC分类号： G01N033/542 ,  B05D003/00 ,  C12M001/34 ,  G01N033/53

CPC分类号： G01N33/5432 ,  G01N33/544

摘要： The present invention relates to a method of preparing a substrate surface supporting a lipid film membrane structure, which method comprises the steps of contacting a substrate surface with an aqueous liquid containing detergent/lipid mixed micelles to adhere detergent/lipid mixed micelles to the substrate surface, and then contacting the substrate surface having detergent/lipid mixed micelles adhered thereto with an aqueous liquid substantially free from detergent to elute the detergent molecules from the adhered mixed micelles and make the remaining lipid molecules assemble into a lipid film membrane structure on the substrate surface. The invention also relates to a substrate supporting a lipid film membrane structure as prepared by the method, the use of a substrate supporting a lipid film membrane structure as prepared by the method for molecular interaction studies, and a substrate surface for use in the method.

摘要翻译： 本发明涉及一种制备支持脂质膜膜结构的底物表面的方法，该方法包括使底物表面与含有洗涤剂/脂质混合胶束的含水液体接触以将洗涤剂/脂质混合胶束粘附到基底表面 然后将粘附有洗涤剂/脂质混合胶束的基材表面与基本上不含洗涤剂的水性液体接触，从粘附的混合胶束中洗脱洗涤剂分子，并使剩余的脂质分子组装成基材表面上的脂质膜膜结构 。 本发明还涉及一种支持通过该方法制备的脂质膜结构的底物，通过该分子相互作用研究方法制备的支持脂质膜膜结构的底物的使用以及用于该方法的底物表面。