公开(公告)号：US20040002167A1

公开(公告)日：2004-01-01

申请号：US10400158

申请日：2003-03-26

申请人： Biacore AB

发明人： Karl Andersson ,  Peter Borg

IPC分类号： G01N033/543

CPC分类号： G01N33/54373 ,  G06F19/18 ,  G06F19/24

摘要： A method of analysis wherein molecular interactions at one or more sensing surface areas are detected and respective response curves representing the progress of each interaction with time are produced, and wherein a resulting set of response curves is subjected to a quality assessment procedure which comprises representing the response curves with one or more quality descriptors, applying a quality classification method to the descriptors to find outliers, and removing the outliers. The invention also relates to an analytical system including means for classifying the response curves with regard to quality, a computer program for performing the classification, and a computer program product containing the program.

摘要翻译： 一种分析方法，其中检测一个或多个感测表面区域处的分子相互作用，并且产生表示每次与时间相互作用的进展的各自的响应曲线，并且其中对所得到的一组响应曲线进行质量评估程序，其包括表示 响应曲线与一个或多个质量描述符，向描述符应用质量分类方法以找出异常值，并移除异常值。 本发明还涉及一种分析系统，包括用于对质量的响应曲线进行分类的装置，用于执行分类的计算机程序以及包含该程序的计算机程序产品。

公开(公告)号：US20040248213A1

公开(公告)日：2004-12-09

申请号：US10815166

申请日：2004-03-31

申请人： Biacore AB

发明人： Robert Karlsson ,  Pascale Richalet-Secordel

IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N033/542

CPC分类号： G01N33/5005 ,  G01N33/543 ,  Y10S435/973

摘要： The present invention relates to a method for analysing a cell sample for cell surface-bound or intracellularly bound analytes by providing an array of immobilised specific binding agents for a set of different ligands, where each ligand is specific to a respective cell surface-bound or intracellularly bound analyte, and using the array and the set of ligands in an inhibition type or a direct type assay format to determine cell surface-bound or intracellularly bound analytes in the cell sample. The invention also relates to assay kits for cell characterization.

摘要翻译： 本发明涉及通过提供用于一组不同配体的固定化特异性结合剂的阵列分析细胞表面结合或细胞内结合的分析物的细胞样品的方法，其中每个配体对相应的细胞表面结合或特异性结合 细胞内结合的分析物，并使用阵列和该组配体以抑制型或直接型测定形式来确定细胞样品中细胞表面结合或细胞内结合的分析物。 本发明还涉及用于细胞表征的测定试剂盒。

公开(公告)号：US20020128593A1

公开(公告)日：2002-09-12

申请号：US10001246

申请日：2001-11-01

申请人： Biacore AB

发明人： Stefan Sjolander ,  Thord Hansson

IPC分类号： A61B017/20

CPC分类号： G01N35/1097 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/0689 ,  B01L2300/0874 ,  B01L2400/0655 ,  F16K7/126 ,  F16K7/17 ,  G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  Y10T29/49405 ,  Y10T29/49417 ,  Y10T29/49425 ,  Y10T29/49426 ,  Y10T29/49428 ,  Y10T137/87893

摘要： The present invention discloses a valve integrally associated with a microfluidic transport assembly that is useful for regulating the flow of a liquid sample through an analytical instrument such as, for example, a biosensor. The valve integrally associated with a microfluidic liquid transport assembly, includes: a first rigid layer having substantially planar and opposing first and second surfaces; a second rigid layer having substantially planar and opposing third and fourth surfaces, the third surface of the second rigid layer being substantially coplanar and integrally bonded to the second surface of the first rigid layer; a first passageway defined by a groove, the groove being along the second surface of the first rigid layer and bounded by the third surface of the second rigid layer, the first passageway being adapted to flow a liquid sample therethrough, a valve seat having a substantially planar plateau surface, the valve seat being within the passageway and integrally connected to the first rigid layer such that the plateau surface is substantially planar to and interposed between the first and second surfaces of the first rigid layer; and a flexible membrane opposite the valve seat and integrally associated with a first membrane through hole of the second rigid layer, the flexible membrane having a passageway surface that is either (i) substantially coplanar to the second surface of the first rigid layer when the valve is in an open position, or (ii) bulged with a central portion thereof being substantially coplanar to the plateau surface of the valve seat when the valve is in a closed position. The present invention is also directed to methods of manufacturing of the same.

摘要翻译： 本发明公开了一种与微流体输送组件整体相关联的阀，其可用于通过诸如生物传感器的分析仪器来调节液体样品的流动。 与微流体液体输送组件整体相关联的阀包括：具有基本平坦且相对的第一和第二表面的第一刚性层; 第二刚性层，其具有基本平坦且相对的第三和第四表面，所述第二刚性层的第三表面基本共面并整体地结合到所述第一刚性层的第二表面; 由凹槽限定的第一通道，所述凹槽沿着所述第一刚性层的第二表面并且由所述第二刚性层的第三表面限定，所述第一通道适于使液体样品流过其中，所述阀座具有基本上 平台平台表面，阀座在通道内并且与第一刚性层一体地连接，使得平台表面基本上平面并插入在第一刚性层的第一和第二表面之间; 以及与所述阀座相对并且与所述第二刚性层的第一膜通孔整体相关联的柔性膜，所述柔性膜具有通道表面，所述通道表面（i）当所述阀（i）基本上与所述第一刚性层的第二表面共面时， 处于打开位置，或（ii）当阀处于关闭位置时，其中心部分与阀座的平台表面基本上共面膨胀。 本发明还涉及其制造方法。

公开(公告)号：US20020182717A1

公开(公告)日：2002-12-05

申请号：US10098739

申请日：2002-03-14

申请人： Biacore AB

发明人： Olof Karlsson

IPC分类号： G01N033/542 ,  B05D003/00 ,  C12M001/34 ,  G01N033/53

CPC分类号： G01N33/5432 ,  G01N33/544

摘要： The present invention relates to a method of preparing a substrate surface supporting a lipid film membrane structure, which method comprises the steps of contacting a substrate surface with an aqueous liquid containing detergent/lipid mixed micelles to adhere detergent/lipid mixed micelles to the substrate surface, and then contacting the substrate surface having detergent/lipid mixed micelles adhered thereto with an aqueous liquid substantially free from detergent to elute the detergent molecules from the adhered mixed micelles and make the remaining lipid molecules assemble into a lipid film membrane structure on the substrate surface. The invention also relates to a substrate supporting a lipid film membrane structure as prepared by the method, the use of a substrate supporting a lipid film membrane structure as prepared by the method for molecular interaction studies, and a substrate surface for use in the method.

摘要翻译： 本发明涉及一种制备支持脂质膜膜结构的底物表面的方法，该方法包括使底物表面与含有洗涤剂/脂质混合胶束的含水液体接触以将洗涤剂/脂质混合胶束粘附到基底表面 然后将粘附有洗涤剂/脂质混合胶束的基材表面与基本上不含洗涤剂的水性液体接触，从粘附的混合胶束中洗脱洗涤剂分子，并使剩余的脂质分子组装成基材表面上的脂质膜膜结构 。 本发明还涉及一种支持通过该方法制备的脂质膜结构的底物，通过该分子相互作用研究方法制备的支持脂质膜膜结构的底物的使用以及用于该方法的底物表面。

公开(公告)号：US20020154311A1

公开(公告)日：2002-10-24

申请号：US10098740

申请日：2002-03-14

申请人： Biacore AB

发明人： Bengt Ivarsson

IPC分类号： G01N021/55

CPC分类号： G01N21/552 ,  G01N21/553 ,  G01N21/648

摘要： An optical apparatus for total internal reflection spectroscopy comprises: a transparent body having an internally reflective surface; at least one source of electromagnetic radiation for providing at least one beam of collimated electromagnetic radiation; optical scanning means for directing the beam or beams to the transparent body so that the radiation is internally reflected at the reflective surface, and sequentially or continuously scanning the incident angle of the radiation over an angular range; at least one detector for detecting electromagnetic radiation exiting the transparent body, and means for counteracting variation of the irradiance in the illuminated area of the surface during the angular scan, or the effect of such variation on the reflected beam or beams. An optical apparatus for examining thin layer structures on a surface for differences in respect of optical thickness and/or refractive index, and a method for total internal reflection spectroscopy are also disclosed.

摘要翻译： 用于全内反射光谱的光学装置包括：具有内部反射表面的透明体; 用于提供至少一个准直电磁辐射束的至少一个电磁辐射源; 用于将光束或光束引导到透明体的光学扫描装置，使得辐射在反射表面处被内部反射，并且在角度范围内依次或连续地扫描辐射的入射角; 用于检测离开透明体的电磁辐射的至少一个检测器，以及用于在角扫描期间抵消表面的照射区域中的辐照度变化的装置，或这种变化对反射光束或束的影响的装置。 还公开了一种用于检查表面上关于光学厚度和/或折射率的差异的薄层结构的光学装置，以及全内反射光谱法的方法。

公开(公告)号：US20040166549A1

公开(公告)日：2004-08-26

申请号：US10722061

申请日：2003-11-25

申请人： Biacore AB

发明人： Robert Karlsson ,  Helena Nordin ,  Susanna Nyberg

IPC分类号： G01N033/53 ,  G01N033/537 ,  G01N033/543

CPC分类号： G01N33/54306 ,  G01N33/54393

摘要： The invention relates to a method of determining the binding site specificity of an analyte to a ligand having at least two different binding sites, which method comprises immobilizing the ligand to a solid support, mixing the analyte with a reversibly binding reference analyte which has a dissociation behaviour that differs significantly from that of the analyte, contacting the mixture with the ligand to determine dissociation characteristics for the mixture, and determining therefrom the binding site specificity of the analyte in relation to the reference analyte. The invention also relates to an analytical system for carrying out the method, and to a computer program, computer program product and computer system for performing the method.

摘要翻译： 本发明涉及一种确定分析物对具有至少两个不同结合位点的配体的结合位点特异性的方法，该方法包括将配体固定在固体支持物上，将分析物与可分离结合的参考分析物混合，该分析物具有解离 与分析物显着不同的行为，使混合物与配体接触以确定混合物的解离特性，并从其中确定分析物相对于参考分析物的结合位点特异性。 本发明还涉及用于执行该方法的分析系统，以及用于执行该方法的计算机程序计算机程序产品和计算机系统。

公开(公告)号：US20040058456A1

公开(公告)日：2004-03-25

申请号：US10449823

申请日：2003-05-30

申请人： Biacore AB

发明人： Par Safsten ,  Mattias Tidare

IPC分类号： G01N033/543 ,  B05D003/00

CPC分类号： B01L3/502776 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00704 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L2200/0636 ,  B01L2300/0636 ,  B01L2300/0877 ,  B82Y30/00 ,  C40B40/10

摘要： The present invention relates to a method of coupling multiple binding agents to respective areas of a substrate surface by hydrodynamic addressing, using two laminar fluid flows that flow together in the same direction over the substrate surface with an interface to each other to successively couple the binding agents to the substrate areas, wherein each successive coupling of a binding agent to a surface area is followed or preceded by selective deactivation or activation of a selected surface area according to a defined protocol. The invention also relates to the use of such a binding agent-coupled substrate surface for analytical purposes.

摘要翻译： 本发明涉及一种通过流体力学寻址将多个粘合剂偶联到基底表面的相应区域的方法，使用两个层流体流，其以相同的方向在相同的方向上在彼此之间的界面相互连接以连续地结合 试剂到基底区域，其中粘合剂与表面区域的每个连续偶联遵循或之前是根据所定义的方案选择性地去活化或选择的表面区域的活化。 本发明还涉及用于分析目的的这种结合剂偶联的底物表面的用途。

公开(公告)号：US20030077842A1

公开(公告)日：2003-04-24

申请号：US10295709

申请日：2002-11-15

申请人： Biacore AB

发明人： Magnus Malmqvist ,  Osten Jansson

IPC分类号： C12Q001/68 ,  G01N033/543

CPC分类号： G01N33/537 ,  G01N21/553 ,  G01N21/648 ,  G01N33/543 ,  G01N33/54313

摘要： Methods for capturing analytes associated with surface-bound ligands are disclosed. The methods involve eluting analytes from surface-bound ligands with a first liquid to generate free analytes, and capturing the free analytes with a solid capturing material within the first liquid to generate a first liquid containing captured analytes. The first liquid may be a flowing liquid or a non-flowing liquid, and the surface to which the surface-bound ligand is attached may be a sensing surface, such as a biosensor, or a non-sensing surface. The captured analytes may be further consolidated at a location removed from the surface-bound ligand, eluted from the solid capturing material with a second liquid, and used for subsequent analysis or procedures.

公开(公告)号：US20030067612A1

公开(公告)日：2003-04-10

申请号：US10244819

申请日：2002-09-16

申请人： Biacore AB

发明人： Bengt Ivarsson

IPC分类号： G01B011/30

CPC分类号： G01B11/0625 ,  G01N21/552 ,  G01N21/553 ,  G01N2021/212 ,  G01N2021/215

摘要： A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.

公开(公告)号：US20040241724A1

公开(公告)日：2004-12-02

申请号：US10806028

申请日：2004-03-22

申请人： Biacore AB

发明人： Robert Karlsson ,  Anders Sjodin

IPC分类号： C12Q001/68 ,  C12M001/34 ,  B05D003/00

CPC分类号： G01N33/5432 ,  G01N33/548

摘要： A method of immobilizing a target molecule to a solid support surface capable of interacting with the target molecule is disclosed. The method comprises the steps of complexing the target molecule with a vesicular structure capable of forming a dissociable complex with the target molecule, contacting the complex formed with the solid support surface to thereby bind the target molecule to the surface, dissociating the complex, and removing the vesicular structure from the solid support surface to leave the target molecule immobilized on the surface. A method of sensitizing a solid support surface, the use of the sensitized solid support surface for analyzing analytes, and a reagent kit for carrying out the method are also disclosed.

摘要翻译： 公开了将目标分子固定在能够与靶分子相互作用的固体支持体表面上的方法。 该方法包括以下步骤：使目标分子与能够与靶分子形成可离解复合物的囊泡结构相接触，使与固体支持物表面形成的复合物接触，从而将靶分子结合到表面，解离复合物，并除去 来自固体支持体表面的水泡结构，使靶分子固定在表面上。 还公开了使固体支持体表面敏化的方法，使用敏化固体支持体表面分析分析物，以及用于实施该方法的试剂盒。