公开(公告)号：US09309281B2

公开(公告)日：2016-04-12

申请号：US13973130

申请日：2013-08-22

申请人： Bigtec Private Limited

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： A61K38/28 ,  C07K14/62 ,  C07K1/18

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus Pichia. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.

摘要翻译： 在一个方面，方法涉及重组DNA治疗领域。 方法可涉及生物信息学设计，人前体肽编码序列的人胰岛素前体的合成，将人工基因克隆到表达载体中，并在生物体中表达，例如选自毕赤酵母属（Pichia）的生物。 在另一方面，方法可以包括用于获得蛋白质前体分子和随后将蛋白质前体分子转化为功能性蛋白质的下游处理。

公开(公告)号：US09238674B2

公开(公告)日：2016-01-19

申请号：US13973102

申请日：2013-08-22

申请人： Bigtec Private Limited

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07K1/18 ,  C07K1/22 ,  C12N15/10 ,  C12N15/81 ,  C12N1/18 ,  C12N1/19 ,  C07K14/62 ,  C07K1/00

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus Pichia. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.

摘要翻译： 在一个方面，方法涉及重组DNA治疗领域。 方法可涉及生物信息学设计，人前体肽编码序列的人胰岛素前体的合成，将人工基因克隆到表达载体中，并在生物体中表达，例如选自毕赤酵母属（Pichia）的生物。 在另一方面，方法可以包括用于获得蛋白质前体分子和随后将蛋白质前体分子转化为功能性蛋白质的下游处理。

公开(公告)号：US20140057318A1

公开(公告)日：2014-02-27

申请号：US13973130

申请日：2013-08-22

申请人： Bigtec Private Limited

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07K14/62

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

公开(公告)号：US20140051842A1

公开(公告)日：2014-02-20

申请号：US13973102

申请日：2013-08-22

申请人： BIGTEC PRIVATE LIMITED

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07K1/18

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

摘要翻译： 本公开涉及重组DNA治疗领域。 它包括生物信息学设计，人胰岛素前体的人造基因的合成，包括前导肽编码序列，表达载体中的克隆和生物体中优选巴斯德毕赤酵母表达。 本公开还涉及用于获得蛋白质前体分子和随后将前体分子转化成功能性蛋白质的下游处理方法。