公开(公告)号：US20230392198A1

公开(公告)日：2023-12-07

申请号：US18324885

申请日：2023-05-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Congcong MA ,  Duc DO ,  Douglas GREINER

IPC: C12Q1/6844 ,  C12N9/12 ,  C12Q1/6876

CPC classification number: C12Q1/6844 ,  C12N9/1276 ,  C12Q1/6876

Abstract: Method and system for detecting reverse transcriptase activity of a sample by digital assay. In an exemplary method, a reaction mixture including the sample, an RNA polymer, and reagents for reverse transcription may be prepared. Complementary DNA (cDNA) may be synthesized in the reaction mixture using the RNA polymer as a template. An amount of the cDNA synthesized may be proportional to the reverse transcriptase activity of the sample. Partitions may be formed after synthesizing the cDNA. The partitions may contain copies of the cDNA at partial occupancy. Each partition may include a portion of the reaction mixture. A target representing the cDNA may be amplified in the partitions. Amplification data for the target may be collected from the partitions.