公开(公告)号：US20040002095A1

公开(公告)日：2004-01-01

申请号：US10394492

申请日：2003-03-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jason Liu ,  John Wesley Breneman III ,  Arlene Carillo ,  Craig Hixson

IPC: C12Q001/68 ,  C07H021/04 ,  C12P019/34 ,  C07K016/46

CPC classification number: C12Q1/682 ,  C12Q2525/161 ,  C12Q2525/151 ,  C12Q2563/179

Abstract: The present invention resides in a detection agent that can be used to amplify a detection signal for a target molecule. The detection agent contains a linear first single-stranded nucleic acid attached to a capture moiety that specific binds the target molecule of interest. The first single-stranded nucleic acid includes a plurality of repeat sequences, each of which is non-homopolymeric and can specifically bind to a second single-stranded nucleic acid that has at least one detectable label. Related method for using the claimed detection agent to amplify a detection signal and a kit for this purpose are also disclosed.

Abstract translation: 本发明涉及可用于放大靶分子检测信号的检测剂。 检测剂含有连接到特异性结合感兴趣的靶分子的捕获部分的线性第一单链核酸。 第一单链核酸包括多个重复序列，每个重复序列均为非均聚物并且可以特异性结合至少具有至少一个可检测标记的第二单链核酸。 还公开了使用所要求保护的检测剂放大检测信号的相关方法和用于此目的的试剂盒。