公开(公告)号：US09914957B2

公开(公告)日：2018-03-13

申请号：US14185744

申请日：2014-02-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Erin Rae Chia

IPC: C12Q1/68 ,  B01L7/00 ,  G01N21/64 ,  B01L3/00

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1872 ,  C12Q1/6844 ,  G01N21/6486 ,  G01N2201/06113

Abstract: A droplet detection system comprises a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel can meet at an intersection. The sample reservoir can include a sample or partition thereof. During use, an emulsion comprising one or more droplets can be generated at the intersection. The emulsion flows from the intersection along a detection channel to a collection reservoir. A detection assembly that is coupled to at least a portion of the detection channel is configured to detect a signal from the one or more droplets. An energy providing member can be in thermal communication with at least one of the carrier fluid reservoir, the sample reservoir, the intersection, the detection channel and the detection assembly. The energy providing member is configured to transfer energy to the emulsion.

公开(公告)号：US20150017648A1

公开(公告)日：2015-01-15

申请号：US14185744

申请日：2014-02-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Erin Rae Chia

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1872 ,  C12Q1/6844 ,  G01N21/6486 ,  G01N2201/06113

Abstract: A droplet detection system comprises a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel can meet at an intersection. The sample reservoir can include a sample or partition thereof. During use, an emulsion comprising one or more droplets can be generated at the intersection. The emulsion flows from the intersection along a detection channel to a collection reservoir. A detection assembly that is coupled to at least a portion of the detection channel is configured to detect a signal from the one or more droplets. An energy providing member can be in thermal communication with at least one of the carrier fluid reservoir, the sample reservoir, the intersection, the detection channel and the detection assembly. The energy providing member is configured to transfer energy to the emulsion.

Abstract translation: 液滴检测系统包括与载体流体储存器流体连通的第一通道和与样品储存器流体连通的第二通道。 第一个通道和第二个通道可以在十字路口相遇。 样品储存器可以包括其样品或分隔物。 在使用期间，可以在交叉点处产生包含一个或多个液滴的乳液。 乳液从检测通道的交叉口流向收集池。 耦合到所述检测通道的至少一部分的检测组件被配置为检测来自所述一个或多个液滴的信号。 能量提供构件可以与载体流体储存器，样品储存器，交点，检测通道和检测组件中的至少一个热连通。 能量提供构件被配置成将能量转移到乳液。