公开(公告)号：US20170146531A1

公开(公告)日：2017-05-25

申请号：US15410930

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael I. Watkins ,  Gregory A. Marr ,  Xiaoyun Yang ,  Richard Bruehl ,  Daming Shan ,  Patrick F. Coleman

IPC: G01N33/564 ,  C07K14/415

CPC classification number: G01N33/564 ,  C07K14/415 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/70 ,  C12N9/1044 ,  G01N2333/415 ,  G01N2333/91085 ,  G01N2800/24

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.