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52 条记录 (耗时 0.021 秒)

    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN
    3.
    发明申请
    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN 审中-公开
    重组体灭活的GLIADIN抗原

    公开(公告)号：US20090311727A1

    公开(公告)日：2009-12-17

    申请号：US12425605

    申请日：2009-04-17

    申请人： MICHAEL I. WATKINS ,  GREGORY A. MARR ,  XIAOYUN YANG ,  RICHARD BRUEHL ,  DAMING SHAN ,  PATRICK F. COLEMAN

    发明人： MICHAEL I. WATKINS ,  GREGORY A. MARR ,  XIAOYUN YANG ,  RICHARD BRUEHL ,  DAMING SHAN ,  PATRICK F. COLEMAN

    IPC分类号： G01N33/53 ,  C07K14/415 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

    CPC分类号： G01N33/564 ,  C07K14/415 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/70 ,  C12N9/1044 ,  G01N2333/415 ,  G01N2333/91085 ,  G01N2800/24

    摘要： The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

    摘要翻译： 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过经由标签固定在固体支持物上的麦醇溶蛋白融合蛋白来制备抗原。 抗原可以进一步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。

    Preparation of factor XIIIa by gene manipulation
    4.
    发明授权
    Preparation of factor XIIIa by gene manipulation 失效
    通过基因操作制备因子XIIIa

    公开(公告)号：US06746866B1

    公开(公告)日：2004-06-08

    申请号：US08460206

    申请日：1995-06-02

    申请人： Ulrich Grundmann ,  Egon Amann ,  Gerd Zettlmeissl

    发明人： Ulrich Grundmann ,  Egon Amann ,  Gerd Zettlmeissl

    IPC分类号： C07H1700

    CPC分类号： C12N9/1044 ,  A61K38/00 ,  C07K16/40 ,  C12Q1/6876 ,  G01N33/86 ,  G01N2333/9108 ,  G01N2333/91085

    摘要： The cDNA which codes for factor XIIIa has been isolated using a cDNA bank from human placenta and probes constructed on the basis of the amino acid sequence of factor XIIIa peptide fragments. It is possible with this cDNA not only to obtain factor XIIIa by gene manipulation in high purity but also to prepare diagnostic aids which permit the analysis of genetic factor XIIIa defects. Furthermore, it is possible on the basis of the amino acid sequence to prepare antibodies which are suitable for diagnostic aids and antibody columns.

    摘要翻译： 使用来自人胎盘的cDNA库和基于因子XIIIa肽片段的氨基酸序列构建的探针分离了编码因子XIIIa的cDNA。 该cDNA不仅可以通过高纯度的基因操作获得因子XIIIa，而且可以制备能够分析遗传因子XIIIa缺陷的诊断辅助物质。 此外，可以基于氨基酸序列制备适用于诊断辅助剂和抗体柱的抗体。

    Biomarkers and targets for diagnosis, prognosis and management of prostate disease
    5.
    发明申请
    Biomarkers and targets for diagnosis, prognosis and management of prostate disease 审中-公开
    用于诊断，预后和前列腺疾病管理的生物标志物和靶标

    公开(公告)号：US20010007748A1

    公开(公告)日：2001-07-12

    申请号：US09756976

    申请日：2001-01-09

    发明人： Gang An ,  Robert W. Vertri

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  G01N033/53

    CPC分类号： C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/136 ,  G01N33/57434 ,  G01N2333/47 ,  G01N2333/4742 ,  G01N2333/9108 ,  G01N2333/91085 ,  Y10S435/81

    摘要： Disclosed are diagnostic techniques for the detection of human prostate disease. The invention relates particularly to probes and methods for evaluating the presence of RNA species that are differentially expressed in metastatic prostate cancer compared to normal human prostate, benign prostatic hyperplasia, and non-metastatic prostate cancer. The invention also relates to probes and methods for evaluating the presence of RNA species that are differentially expressed in the peripheral blood of individuals with the disease state compared to normal healthy individuals. Described are methods of therapeutic use for genes identified as differentially expressed in metastatic prostate cancer, and means for screening pharmaceuticals effective in treatment of prostate cancer.

    摘要翻译： 公开了用于检测人前列腺疾病的诊断技术。 本发明特别涉及用于评估与正常人前列腺，良性前列腺增生和非转移性前列腺癌相比在转移性前列腺癌中差异表达的RNA物质的存在的探针和方法。 本发明还涉及用于评估与正常健康个体相比具有疾病状态的个体的外周血中差异表达的RNA物种的存在的探针和方法。 描述了在转移性前列腺癌中鉴定为差异表达的基因的治疗用途的方法，以及用于筛选有效治疗前列腺癌的药物的方法。

    Pet leash with length-limited elastic section
    6.
    发明授权
    Pet leash with length-limited elastic section 失效
    宠物皮带长度有限弹性部分

    公开(公告)号：US5873328A

    公开(公告)日：1999-02-23

    申请号：US10393

    申请日：1998-01-21

    申请人： William E. Campbell

    发明人： William E. Campbell

    IPC分类号： A01K27/00 ,  C12Q1/68 ,  G01N33/574

    CPC分类号： A01K27/005 ,  C12Q1/6809 ,  G01N33/57434 ,  G01N2333/47 ,  G01N2333/4742 ,  G01N2333/91085

    摘要： A leash assembly for training dogs includes a handle and an elongate strap connected at one end to the handle, the other end being adapted to be connected to a collar worn by the dog. An elastic element has a first length when in a relaxed state and is extensible four inches to a second length when fully extended. The elastic element is attached to the strap with the length of strap between attached ends of the elastic element being substantially equal to the second length. The force required to reach the fully extended state is about 5 pounds or 2.3 kilograms. The elastic element significantly limits the impact of strong, short correctional tugs or jerks on the leash by the trainer.

    摘要翻译： 用于训练狗的皮带组件包括手柄和在一端连接到手柄的细长带，另一端适于连接到由狗佩戴的衣领。 当处于松弛状态时，弹性元件具有第一长度，并且当完全伸展时，弹性元件可伸长4英寸至第二长度。 弹性元件附接到带子，弹性元件的附接端之间的带的长度基本上等于第二长度。 达到完全伸展状态所需的力约为5磅或2.3公斤。 弹性元素显着地限制了训练师对强力，短暂的矫正拖轮或冲刺对皮带的影响。

    Method of determining blood coagulation factor XIII activity and kit of reagents for the determination
    7.
    发明授权
    Method of determining blood coagulation factor XIII activity and kit of reagents for the determination 失效
    确定凝血因子XIII活性的方法和试剂盒用于测定

    公开(公告)号：US5508202A

    公开(公告)日：1996-04-16

    申请号：US232221

    申请日：1994-05-04

    申请人： Masayasu Enomoto ,  Masahiro Yamaguchi

    发明人： Masayasu Enomoto ,  Masahiro Yamaguchi

    IPC分类号： C12Q1/56 ,  G01N33/86

    CPC分类号： C12Q1/56 ,  G01N2333/75 ,  G01N2333/9108 ,  G01N2333/91085 ,  G01N2333/974

    摘要： An accurate, rapid and simple method of determining blood coagulation factor XIII activity and a kit of reagents therefor are provided. A sample and a fibrin precipitation inhibitor are mixed, or a sample, fibrinogen and a fibrin precipitation inhibitor are mixed; a thrombin solution is added; the fibrin coagulation time is measured in the presence of calcium ion; and the coagulation time is compared with the normal coagulation time. The kit for the determination comprises thrombin, calcium ion and a fibrin precipitation inhibitor, and may be combined with fibrinogen.

    摘要翻译： PCT No.PCT / JP93 / 01247 Sec。 371日期：1994年5月4日 102（e）日期1994年5月4日PCT 1993年9月3日PCT公布。 公开号WO94 / 07145 日期为1994年3月31日。提供了一种准确，快速和简单的测定凝血因子XIII活性的方法及其试剂盒。 将样品和纤维蛋白沉淀抑制剂混合，或将样品，纤维蛋白原和纤维蛋白沉淀抑制剂混合; 加入凝血酶溶液; 在钙离子存在下测量纤维蛋白凝固时间; 并将凝固时间与正常凝固时间进行比较。 用于测定的试剂盒包括凝血酶，钙离子和纤维蛋白沉淀抑制剂，并且可以与纤维蛋白原组合。