公开(公告)号：US10787694B2

公开(公告)日：2020-09-29

申请号：US15977254

申请日：2018-05-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  John Sullivan ,  Rongdian Fu ,  Yan Wang ,  Evan H. Bursey ,  Man Cheng

IPC: C07K19/00 ,  C12Q1/6804 ,  C12Q1/686 ,  C12P19/34 ,  C07K16/40 ,  C12N9/12

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

公开(公告)号：US20140377767A1

公开(公告)日：2014-12-25

申请号：US14482756

申请日：2014-09-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  John Sullivan ,  Rongdian Fu ,  Yan Wang ,  Evan H. Bursey ,  Man Cheng

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12P19/34 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6804 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/101

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Abstract translation: 本发明提供了提高核酸扩增反应效率的方法和组合物。 本发明包括与野生型聚合酶相比显示增加的活性以及降低的核酸外切酶活性的杂合聚合酶。 本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。

公开(公告)号：US09994887B2

公开(公告)日：2018-06-12

申请号：US14482756

申请日：2014-09-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  John Sullivan ,  Rongdian Fu ,  Yan Wang ,  Evan H. Bursey ,  Man Cheng

IPC: C12Q1/68 ,  C12P19/34 ,  C07K16/40 ,  C12N9/12

CPC classification number: C12P19/34 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6804 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/101

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.