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    miRNA transcriptome methods and compositions
    2.
    发明授权
    miRNA transcriptome methods and compositions 有权

    公开(公告)号：US10781481B2

    公开(公告)日：2020-09-22

    申请号：US15499734

    申请日：2017-04-27

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Rongdian Fu

    IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6876 ,  C12N15/66 ,  C12Q1/6806

    摘要： Methods, polynucleotides, kits, and reaction mixtures are disclosed for the enriching of short polynucleotide molecules that have a length within a desired target length range. A Type IIS or Type III restriction enzyme is used to cleave polynucleotides at cleavage sites located at a distance from the restriction enzyme recognition sites. For example, a mixture of polynucleotides can be formed by inserting DNA molecules between a recognition site for the restriction enzyme and a region of non-naturally-occurring nucleotides that block cleavage by the restriction enzymes. If a polynucleotide contains a DNA molecule with a length within a target range, then the cleavage site will be within the blocking region, and cleavage will not occur. Polynucleotides containing DNA molecules with lengths outside the target range can be cleaved. By selectively enriching, through PCR or other means, polynucleotides that are intact, a concentrated population of polynucleotides of a target length can be formed.