公开(公告)号：US20150072351A1

公开(公告)日：2015-03-12

申请号：US14391155

申请日：2013-04-08

申请人： Bioneer Corporation

发明人： Han Oh Park ,  Jun Hee Lee ,  Hyun Seo Kim ,  Sora Choi ,  Jong Hoon Kim

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6848 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2525/186 ,  C12Q2531/113

摘要： The present invention relates to a method for preparing a nucleic acid with high sensitivity, wherein a nucleic acid polymerase is used to add a terminator to the nucleic acid to be used for analysis prior to a nucleic acid polymerization reaction such as a PCR reaction, a real time quantitative PCR reaction, or the like, for detecting a trace of nucleic acid, such that a non-specific priming occurring competitively with an amplification reaction of a target nucleic acid may be basically eliminated, thereby precisely detecting only the trace of target nucleic acid and precisely measuring a concentration of the target nucleic acid.

摘要翻译： 本发明涉及一种制备具有高灵敏度的核酸的方法，其中使用核酸聚合酶将核酸聚合反应如PCR反应之前用于分析的核酸加入终止子， 实时定量PCR反应等，用于检测核酸的痕迹，使得可以基本上消除与目标核酸的扩增反应竞争性发生的非特异性引发，从而精确检测目标核酸的痕迹 酸并精确测量靶核酸的浓度。