公开(公告)号：US20250019754A1

公开(公告)日：2025-01-16

申请号：US18769390

申请日：2024-07-11

Applicant: Biotaitan Molmed Co. Ltd.

Inventor： Ching-Wei TSAI ,  Yih-Jyh SHANN ,  Szu-Yu LIU ,  Li-Chi CHANG

IPC: C12Q1/6851 ,  C12Q1/6818 ,  C12Q1/6876

Abstract: A method for detecting multiple targets in the same sample includes the following steps. A sample is provided, and the sample includes a nucleic acid of at least one target. A reaction solution is provided, which includes target primer pairs and target fluorescent probes. The target fluorescent probes include at least two fluorophores. After mixing the sample and the reaction solution, a real-time polymerase chain reaction is performed. A fluorescence signal value ratio of the at least two fluorophores is calculated to determine whether the at least one target is present in the sample.