公开(公告)号：US09993544B2

公开(公告)日：2018-06-12

申请号：US15312249

申请日：2015-05-22

申请人： Boehringer Ingelheim Vetmedica GmbH

发明人： Gregor Meyers ,  Sabine Wirtz

IPC分类号： A61K39/12 ,  G01N33/569 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/24321 ,  C12N2770/24322 ,  C12N2770/24334 ,  C12N2770/24362 ,  G01N33/56983 ,  G01N2333/185

摘要： The present invention relates i.a. to a CSFV (classical swine fever virus) comprising a substitution of proline to lysine at amino acid position 44 of the E2 protein and a substitution of threonine to aspartic acid at amino acid position 45 of the E2 protein. Further, the present invention provides an immunogenic composition comprising the CSFV of the present invention and the use of the immunogenic composition for reducing the incidence of or severity in an animal of one or more clinical signs associated with CSF. Moreover, the present invention provides a method of differentiating animals infected with CSFV from animals vaccinated with the immunogenic composition of the present invention.