公开(公告)号：US5712113A

公开(公告)日：1998-01-27

申请号：US353751

申请日：1994-12-12

申请人： Bong Hyun Chung ,  Soo Wan Nam ,  Byung Moon Kim ,  Sun Ah Yang ,  Young Hoon Park

发明人： Bong Hyun Chung ,  Soo Wan Nam ,  Byung Moon Kim ,  Sun Ah Yang ,  Young Hoon Park

IPC分类号： C12N15/09 ,  C07K14/47 ,  C12N1/19 ,  C12N9/24 ,  C12N15/00 ,  C12N15/56 ,  C12N15/81 ,  C12P21/02 ,  C12R1/645 ,  C12R1/865 ,  C12P21/06

CPC分类号： C12Y302/01007 ,  C07K14/4721 ,  C12N15/81 ,  C12N15/815 ,  C12N9/2402 ,  C07K2319/00 ,  C07K2319/02

摘要： Secretion signal peptides of inulinases derived from Kluyveromyces marxianus, which makes a heterologous protein produced in a yeast cell be secreted almost completely into the extracellular medium; nucleotide sequences encoding said secretion signal peptides; expression and secretion vectors comprising nucleotide sequences encoding said secretion signal peptides; recombinant yeast cells transformed with said expression and secretion vectors; and a process for producing heterologous proteins by culturing said recombinant yeast cells.

摘要翻译： 衍生自马克斯克鲁维酵母的菊粉酶的分泌信号肽，其使得在酵母细胞中产生的异源蛋白质几乎完全分泌到细胞外培养基中; 编码所述分泌信号肽的核苷酸序列; 包含编码所述分泌信号肽的核苷酸序列的表达和分泌载体; 用所述表达和分泌载体转化的重组酵母细胞; 以及通过培养所述重组酵母细胞产生异源蛋白质的方法。