Protein detection and quantitation using hydroxyquinolone dyes
    2.
    发明授权
    Protein detection and quantitation using hydroxyquinolone dyes 有权
    蛋白质检测和定量使用羟基喹诺酮染料

    公开(公告)号:US07947506B2

    公开(公告)日:2011-05-24

    申请号:US11421883

    申请日:2006-06-02

    CPC分类号: G01N33/6839

    摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.

    摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。

    PROTEIN DETECTION AND QUANTITATION USING HYDROXYQUINOLONE DYES
    3.
    发明申请
    PROTEIN DETECTION AND QUANTITATION USING HYDROXYQUINOLONE DYES 有权
    蛋白质检测和定量使用羟基喹啉染料

    公开(公告)号:US20070281360A1

    公开(公告)日:2007-12-06

    申请号:US11421883

    申请日:2006-06-02

    IPC分类号: G01N33/00

    CPC分类号: G01N33/6839

    摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.

    摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。

    ELECTROBLOT TRANSFER BUFFER
    5.
    发明申请
    ELECTROBLOT TRANSFER BUFFER 有权
    电子传输缓冲器

    公开(公告)号:US20160266065A1

    公开(公告)日:2016-09-15

    申请号:US15043816

    申请日:2016-02-15

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44747 G01N27/44739

    摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

    摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干转印。

    Electroblot transfer buffer
    6.
    发明授权
    Electroblot transfer buffer 有权
    Electroblot传输缓冲区

    公开(公告)号:US09291596B2

    公开(公告)日:2016-03-22

    申请号:US13764934

    申请日:2013-02-12

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44747 G01N27/44739

    摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

    摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干转印。

    ELECTROBLOT TRANSFER BUFFER
    7.
    发明申请
    ELECTROBLOT TRANSFER BUFFER 有权
    电子传输缓冲器

    公开(公告)号:US20140224655A1

    公开(公告)日:2014-08-14

    申请号:US13764934

    申请日:2013-02-12

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44747 G01N27/44739

    摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

    摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干燥转移。