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公开(公告)号:US07553869B2
公开(公告)日:2009-06-30
申请号:US11460700
申请日:2006-07-28
申请人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
发明人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
IPC分类号: A01N43/16 , A61K31/35 , C07D311/02
CPC分类号: C07D491/052 , C07D311/56 , C07D493/04 , G01N27/44726 , G01N33/6827
摘要: Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.
摘要翻译: 披露了使用该化合物和组合物进行蛋白质检测,测定,定量等的香豆素/氯诺酮和马丁纳型化合物,组合物和方法。 该组合物,也称为染料,具有比商业上可获得的蛋白质污渍更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物的蛋白质等。
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公开(公告)号:US07947506B2
公开(公告)日:2011-05-24
申请号:US11421883
申请日:2006-06-02
申请人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
发明人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
CPC分类号: G01N33/6839
摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.
摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。
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公开(公告)号:US20070281360A1
公开(公告)日:2007-12-06
申请号:US11421883
申请日:2006-06-02
申请人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
发明人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
IPC分类号: G01N33/00
CPC分类号: G01N33/6839
摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.
摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。
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公开(公告)号:US20080026478A1
公开(公告)日:2008-01-31
申请号:US11460700
申请日:2006-07-28
申请人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
发明人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
IPC分类号: G01N33/50 , C07D409/02 , C07D405/02
CPC分类号: C07D491/052 , C07D311/56 , C07D493/04 , G01N27/44726 , G01N33/6827
摘要: Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.
摘要翻译: 披露了使用该化合物和组合物进行蛋白质检测,测定,定量等的香豆素/氯诺酮和马丁纳型化合物,组合物和方法。 该组合物,也称为染料,具有比商业上可获得的蛋白质污渍更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物的蛋白质等。
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公开(公告)号:US20160266065A1
公开(公告)日:2016-09-15
申请号:US15043816
申请日:2016-02-15
申请人: Gregory John Kilmer , Brian David Wolf , Scott Eugene Starwalt , Gary G. Judd , Brian Lynn Webb
发明人: Gregory John Kilmer , Brian David Wolf , Scott Eugene Starwalt , Gary G. Judd , Brian Lynn Webb
IPC分类号: G01N27/447
CPC分类号: G01N27/44747 , G01N27/44739
摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.
摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干转印。
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公开(公告)号:US09291596B2
公开(公告)日:2016-03-22
申请号:US13764934
申请日:2013-02-12
IPC分类号: G01N27/447
CPC分类号: G01N27/44747 , G01N27/44739
摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.
摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干转印。
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公开(公告)号:US20140224655A1
公开(公告)日:2014-08-14
申请号:US13764934
申请日:2013-02-12
申请人: Gregory John Kilmer , Brian David Wolf , Scott Eugene Starwalt , Gary G. Judd , Brian Lynn Webb
发明人: Gregory John Kilmer , Brian David Wolf , Scott Eugene Starwalt , Gary G. Judd , Brian Lynn Webb
IPC分类号: G01N27/447
CPC分类号: G01N27/44747 , G01N27/44739
摘要: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.
摘要翻译: 用于将蛋白质或多肽从聚丙烯酰胺凝胶快速转移到合适的膜如硝酸纤维素或聚偏二氟乙烯(PVDF)的半干式一步电泳转移缓冲液组合物。 该组合物含有最小化电阻的组分,并且能够使用常规容易获得的滤纸即棉纤维素纤维实现高效快速半干燥转移。
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