公开(公告)号：US20240342704A1

公开(公告)日：2024-10-17

申请号：US18505857

申请日：2023-11-09

申请人： Bruker Cellular Analysis, Inc.

发明人： Peter J. Beemiller ,  Alexander J. Mastroianni ,  Shao Ning Pei ,  Randall D. Lowe, Jr. ,  Annamaria Mocciaro ,  Kevin D. Loutherback ,  Yelena Bronevetsky ,  Guido K. Stadler ,  Andrew W. McFarland ,  Kevin T. Chapman ,  Duane Smith ,  Natalie C. Marks ,  Amanda L. Goodsell

IPC分类号： B01L3/00 ,  C07K14/74 ,  G01N33/50 ,  G01N33/68

CPC分类号： B01L3/5027 ,  C07K14/70539 ,  G01N33/5008 ,  G01N33/6845 ,  B01L2300/16 ,  G01N33/505 ,  G01N2333/70539

摘要： Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.