-
1.发明公开公开(公告)号：US20240302378A1
公开(公告)日：2024-09-12
申请号：US18481448
申请日：2023-10-05
发明人： Or Gadish , Kellen C. Mobilia , Sara Tafoya , Eric K. Sackmann , Alexander J. Mastroianni , Peyton Shieh , Smriti Sridhar , Pierre Sphabmixay , Eva Y. Cheung , Grayson T. Wawrzyn , Long V. Le , Nathan J. Ver Heul , Bo Hu , Lily Chao , Ke-Chih Lin , Volker L.S. Kurz , Robert M. Onorato
CPC分类号： G01N33/582 , C12M23/16 , C12M41/46
摘要： Metabolic engineering has developed microbial cell factories as sustainable alternatives to chemical synthesis from petroleum feedstocks or harvesting of animals and plants, but current methods can be a costly and labor-intensive commitment. Methods are described herein for microfluidic methods of screening thousands of variant cells in order to reduce time and uncertainty to provide improved strains.
-
公开(公告)号：US20240318120A1
公开(公告)日：2024-09-26
申请号：US18043262
申请日：2021-08-30
发明人： Patrick N. INGRAM , Alexander CHIEN , Ke-Chih LIN , Or GADISH , Troy A. LIONBERGER , Eric K. SACKMANN , Volker L.S. KURZ , Alexander J. MASTROIANNI , Randall D. LOWE, JR. , Jonathan Cloud Dragon HUBBARD
CPC分类号： C12M41/34 , C12M23/16 , C12M41/06 , C12M41/36 , G01N21/05 , G01N21/6408 , G01N21/6458 , G01N2021/6432 , G01N2021/6482 , G01N2021/7786
摘要： Methods, systems, and kits for determining a level of dissolved oxygen within a microfluidic device are provided. The microfluidic device can be suitable for cell culture. The methods, systems, and kits can further be used to determine a level of oxygen consumption in a population of biological micro-objects. In particular, the methods, systems, and kits of the present disclosure rely on flowing a fluidic medium containing a dye and a supplied partial pressure of oxygen into the microfluidic device for a period of time, wherein fluorescence emitted by the dye changes based on availability of oxygen proximate to the dye; taking a fluorescence image of an area of interest within the chamber; and correlating fluorescence of the fluorescence image of the area of interest to a reference to determine an observed partial pressure of the oxygen in the area of interest.
-
公开(公告)号：US20240342704A1
公开(公告)日：2024-10-17
申请号：US18505857
申请日：2023-11-09
发明人： Peter J. Beemiller , Alexander J. Mastroianni , Shao Ning Pei , Randall D. Lowe, Jr. , Annamaria Mocciaro , Kevin D. Loutherback , Yelena Bronevetsky , Guido K. Stadler , Andrew W. McFarland , Kevin T. Chapman , Duane Smith , Natalie C. Marks , Amanda L. Goodsell
CPC分类号： B01L3/5027 , C07K14/70539 , G01N33/5008 , G01N33/6845 , B01L2300/16 , G01N33/505 , G01N2333/70539
摘要： Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.
-
公开(公告)号：US11993766B2
公开(公告)日：2024-05-28
申请号：US16578026
申请日：2019-09-20
IPC分类号： C12M1/12 , C07K17/02 , C12M1/00 , C12M1/32 , C12N5/0783
CPC分类号： C12M25/06 , C07K17/02 , C12M23/12 , C12M23/20 , C12N5/0636
摘要： In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces of wells within a well plate to activate lymphocytes, including but not limited to T lymphocytes, in a controllable and reproducible manner.
-
-
-
搜索结果
4 条记录 (耗时 0.011 秒)
