公开(公告)号：US20080092252A1

公开(公告)日：2008-04-17

申请号：US10581472

申请日：2004-11-30

申请人： Bruno Philippe Angelo Cammue ,  Miguel Francesco Coleta De Bolle ,  Katleen Butaye

发明人： Bruno Philippe Angelo Cammue ,  Miguel Francesco Coleta De Bolle ,  Katleen Butaye

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N15/822

摘要： Disclosed herein are methods and means of achieving enhanced expression of a target nucleotide sequence in a transgenic organism, which methods comprise the steps of: (i) providing an organism in which post-transcriptional gene silencing (PTGS) is suppressed, (ii) associating said target nucleotide sequence with one or more heterologous Matrix Attachment Region (MARs), and (iii) causing or permitting expression from the target nucleotide sequence in the organism. Unexpectedly, the MARs do not merely relieve gene silencing, but can actually lead to expression levels higher than can be achieved in wild-type organisms and higher than expression levels in organisms in which PTGS is suppressed but where the MARs are not employed.

摘要翻译： 本文公开了在转基因生物体中实现靶核苷酸序列增强表达的方法和方法，该方法包括以下步骤：（i）提供抑制转录后基因沉默（PTGS）的生物体，（ii）缔合 所述靶核苷酸序列具有一个或多个异源基质附着区（MAR），和（iii）引起或允许从生物体中的靶核苷酸序列表达。 意外的是，MAR不仅仅是缓解基因沉默，而且实际上可以导致在野生型生物体中可以达到的表达水平高于PTGS被抑制但没有使用MAR的生物中的表达水平。