公开(公告)号：US12173363B2

公开(公告)日：2024-12-24

申请号：US17863183

申请日：2022-07-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Jacob T. Barlow ,  Said R. Bogatyrev

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

公开(公告)号：US20240084360A1

公开(公告)日：2024-03-14

申请号：US18351461

申请日：2023-07-12

Applicant: California Institute of Technology

Inventor： Rustem F. Ismagilov ,  Natalie Wu-Woods ,  Jacob T. Barlow ,  Anna E. Romano

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Provided herein are methods and systems to selectively deplete a biological sample of host compartments and/or host nucleic acid while enriching the sample microbial compartments and/or related microbial nucleic acid. In addition, provided herein are compositions, methods and systems related to said host depletion and microbial enrichment methods and systems.