公开(公告)号：US20230416815A1

公开(公告)日：2023-12-28

申请号：US18025664

申请日：2021-09-14

Applicant: CAMBRIDGE ENTERPRISE LIMITED

Inventor： Manuel NAPPI ,  Alexandre HOFER ,  Shankar BALASUBRAMANIAN ,  Matthew J. GAUNT

IPC: C12Q1/6874 ,  C12Q1/6818 ,  C07D213/61 ,  C07D213/81 ,  C07D213/75

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q1/6806 ,  C07D213/81 ,  C07D213/75 ,  C07D213/61

Abstract: The invention provides a method for labeling a nucleic acid comprising N6-methyl adenine. The method comprises forming an alpha-amino radical on the N6-methyl group of N6mAde, and capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group. The presence of N6mAde in a nucleic acid may then be established by detection of the labeled nucleic acid, or the labeled nucleic acid may be extracted or further modified using the label. Also provided is a method for mapping the position of N6mAde within a target nucleic acid, and probe molecules and kits for use in the method.