公开(公告)号：US10738289B2

公开(公告)日：2020-08-11

申请号：US15466714

申请日：2017-03-22

Applicant: CELLECTIS S.A.

Inventor： George Silva ,  Marine Beurdeley

IPC: C12N9/22 ,  C07K14/47 ,  C12N9/16 ,  C12N15/90 ,  C12N15/82 ,  C07K14/195

Abstract: The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases I-TevI. More precisely, the invention relates to the deciphering of new preferential I-TevI cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.

公开(公告)号：US20170260513A1

公开(公告)日：2017-09-14

申请号：US15466714

申请日：2017-03-22

Applicant: CELLECTIS S.A.

Inventor： George Silva ,  Marine Beurdeley

IPC: C12N9/22 ,  C07K14/195 ,  C12N15/82 ,  C12N15/90

Abstract: The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases I-TevI. More precisely, the invention relates to the deciphering of new preferential I-TevI cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.