公开(公告)号：US20220065808A1

公开(公告)日：2022-03-03

申请号：US17271164

申请日：2020-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Junyi HE ,  Qin ZHU ,  Chu LIU ,  Saifei FENG ,  Huiying HU ,  Donghong YU

IPC: G01N27/327 ,  G01N27/48 ,  C01B32/174

Abstract: A method for a selective detection of L-tryptophan (L-Trp) using formaldehyde as a medium is disclosed. The method includes preparation of a copper sulfide nanosheets-chitosan/acidified functionalized multi-wall carbon nanotubes (CuS NS-CS/F-MWCNTs) composite material, preparation of a composite film-modified electrode CuS NS-CS/F-MWCNTs/GCE, and a detection of the L-Trp. Since oxidation peaks of L-Trp and L-tyrosine (L-Tyr) overlap and are difficult to separate, the present invention provides a method for a highly selective detection of L-Trp through the Pictet-Spengler reaction of formaldehyde (HCHO) with L-Trp, in which the oxidation peak potential of L-Trp is shifted to 0.82 V and the oxidation peak potential of L-Tyr is 0.63 V, thereby effectively avoiding the interference of L-Tyr. The CuS NS-CS/F-MWCNTs/GCE is applied to detect L-Trp in the formaldehyde medium without any interference from L-Tyr or other amino acids with 50-fold concentrations.