公开(公告)号：US20230101196A1

公开(公告)日：2023-03-30

申请号：US16969588

申请日：2019-12-31

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Ningtao ZHOU ,  Li ZHOU ,  Qin ZHU ,  Jinglin HE ,  Zhongliang XIAO ,  Yumin HE ,  Zemeng FENG ,  Yulong YIN

IPC: G01N27/327 ,  G01N27/48 ,  C07K1/107

Abstract: A method and a sensor for detecting L-arginine are provided. The method includes synthesizing ferrocene-functionalized hexadecapeptide dithiocyclopentane (FC-P16 Peptide), preparing a polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE), detecting L-Arg and other steps. The results show that the polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE) exhibits excellent electrochemical response properties to L-Arg. In 10 mmol/L phosphate-buffered saline (PBS, pH=7.4), the DPV response peak current of the polypeptide composite membrane-modified electrode has an excellent linear relationship with the L-Arg concentration of 1.0×10−13 mol/L to 1.0×10−7 mol/L, with a detection limit of 1.0×10−13 mol/L. With prominent reproducibility, repeatability and selectivity, the modified electrode has potential application in life science and nutritional health.

公开(公告)号：US20210025846A1

公开(公告)日：2021-01-28

申请号：US16955797

申请日：2019-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Yuyu PENG ,  Chen LIU ,  Ling TANG ,  Qin ZHU ,  Min JIN ,  Jinglin HE ,  Zhongliang XIAO

IPC: G01N27/414

Abstract: A method and a sensor for detecting L-cysteine based on a 3,3′-dithiobis (1-propanesulfonate)-mercury composite membrane are provided. The method includes: implanting a p-well and an N-type substrate on field effect transistor substrate, constructing a source electrode and a drain electrode at the p-well, constructing a silicon dioxide layer on the substrate, plating an aluminum-copper alloy layer, a chromium-palladium alloy layer, and a gold film layer on a polysilicon gate substrate, constructing a silicon nitride layer on the substrate and the silicon dioxide layer; extending a gate part; preparing an ethanol solution of disodium 3,3′-dithiobis (1-propanesulfonate), cleaning and soaking gate gold electrode in the solution, followed by soaking in an ethanol solution of mercuric nitrate, and then washing the gate gold electrode; connecting electrode interfaces, inserting the electrode into PBS, and connecting power interfaces to a stabilized voltage supply, connecting a signal output interface to a multimeter.

公开(公告)号：US20220065808A1

公开(公告)日：2022-03-03

申请号：US17271164

申请日：2020-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Junyi HE ,  Qin ZHU ,  Chu LIU ,  Saifei FENG ,  Huiying HU ,  Donghong YU

IPC: G01N27/327 ,  G01N27/48 ,  C01B32/174

Abstract: A method for a selective detection of L-tryptophan (L-Trp) using formaldehyde as a medium is disclosed. The method includes preparation of a copper sulfide nanosheets-chitosan/acidified functionalized multi-wall carbon nanotubes (CuS NS-CS/F-MWCNTs) composite material, preparation of a composite film-modified electrode CuS NS-CS/F-MWCNTs/GCE, and a detection of the L-Trp. Since oxidation peaks of L-Trp and L-tyrosine (L-Tyr) overlap and are difficult to separate, the present invention provides a method for a highly selective detection of L-Trp through the Pictet-Spengler reaction of formaldehyde (HCHO) with L-Trp, in which the oxidation peak potential of L-Trp is shifted to 0.82 V and the oxidation peak potential of L-Tyr is 0.63 V, thereby effectively avoiding the interference of L-Tyr. The CuS NS-CS/F-MWCNTs/GCE is applied to detect L-Trp in the formaldehyde medium without any interference from L-Tyr or other amino acids with 50-fold concentrations.

公开(公告)号：US20210123093A1

公开(公告)日：2021-04-29

申请号：US17258932

申请日：2019-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong CAO ,  Jia YANG ,  Chen LIU ,  Zhongliang XIAO ,  Dan LI ,  Ling ZHANG ,  Yuyang ZHANG ,  Jiaxin LI

IPC: C12Q1/6809 ,  G01N27/26

Abstract: A method and a sensor for detecting L-cystine are disclosed. The method is implemented by assembling a sodium 3,3′-dithiodipropane sulfonate (SPS) membrane on a surface of Au membrane layer of an Au electrode and using an extended gate of field effect transistor (FET) and in-situ signal amplification of the FET to detect L-cystine sensitively. The polyanion of the SPS membrane adsorbs and binds a positively charged target L-cystine through electrostatic interaction, thus forming an electric double layer structure to generate a membrane potential identifying a monovalent organic ammonium ion. The sensor includes the FET, wherein a gate-extended gold electrode is arranged on the FET, and the SPS membrane is assembled on the surface of the Au membrane layer of the gate-extended gold electrode. The sensor has an excellent Nernst response to L-cystine.